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Anti human vegf receptor 2 and phospho vegf receptor 2

Manufactured by Cell Signaling Technology

Anti-human VEGF receptor 2 and phospho-VEGF receptor 2 are antibodies that detect the vascular endothelial growth factor (VEGF) receptor 2 and its phosphorylated form, respectively. These reagents are used to study the expression and activation of VEGF receptor 2, a key signaling protein involved in angiogenesis and vascular development.

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2 protocols using anti human vegf receptor 2 and phospho vegf receptor 2

1

Quantitative Protein Analysis of Lung Tissue

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Western blot was performed on total lung homogenates prepared in lysis buffer containing protease and phosphatase inhibitors as previously described (19 (link)) using the following antibodies: rabbit polyclonal EC-SOD antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal mouse endothelial nitric oxide synthase (eNOS) antibody (1: 500, BD Biosciences, San Jose CA), rabbit polyclonal VEGF antibody (1:500, Santa Cruz Biotechnology), rabbit monoclonal Anti-human VEGF receptor 2 and phospho-VEGF receptor 2 (1:1000, Cell Signaling, Danvers, MA), EC-SOD ( 1:1000, Santa Cruz Biotechnology) and B-actin mouse monoclonal antibody (Sigma). The species-appropriate secondary IgG antibody was used (1:10,000, Millipore, Billerica, MA).
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2

Quantitative Protein Analysis of Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot was performed on total lung homogenates prepared in lysis buffer containing protease and phosphatase inhibitors as previously described (19 (link)) using the following antibodies: rabbit polyclonal EC-SOD antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal mouse endothelial nitric oxide synthase (eNOS) antibody (1: 500, BD Biosciences, San Jose CA), rabbit polyclonal VEGF antibody (1:500, Santa Cruz Biotechnology), rabbit monoclonal Anti-human VEGF receptor 2 and phospho-VEGF receptor 2 (1:1000, Cell Signaling, Danvers, MA), EC-SOD ( 1:1000, Santa Cruz Biotechnology) and B-actin mouse monoclonal antibody (Sigma). The species-appropriate secondary IgG antibody was used (1:10,000, Millipore, Billerica, MA).
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