Mice were anesthetized using an isoflurane vaporizer (Kent Scientific) and placed in a stereotaxic frame (David Kopf Instruments) with a Cunningham adaptor (Harvard Apparatus) while a small incision was made and a hole drilled overlying the medial forebrain bundle. A calibrated glass micropipette (Drummond Scientific Company) pulled on a P-97 micropipette puller (Sutter Instruments) was filled with 0.02% ascorbic acid in sterile saline (vehicle) or 6-OHDA (3.5 μg in vehicle) was lowered into the medial forebrain bundle (AP: −0.7, ML: 1.2, DV: −4.75) and 1 μl injected. After stereotaxic injection mice were monitored and provided saline injections and/or high-fat/high-sucrose food on an as needed basis.
Isoflurane vaporizer
Manufactured by Kent Scientific
Sourced in United States
The Isoflurane vaporizer is a device used to precisely control the concentration of isoflurane, a commonly used inhalation anesthetic, during medical procedures. The vaporizer accurately dispenses the desired amount of isoflurane into the anesthetic gas stream, allowing for precise and consistent delivery to the patient.
Automatically generated - may contain errors
Lab products found in correlation
Stereotaxic frame, by Kopf Instruments (1 mentions)
P 97 micropipette puller, by Sutter Instruments (1 mentions)
Cunningham adaptor, by Harvard Apparatus (1 mentions)
Isoflurane, by Kent Scientific (1 mentions)
Micron 4 fundus camera, by Phoenix Pharmaceuticals (1 mentions)
Xlpln25xsvmp2, by Olympus (1 mentions)
Trim scope 2, by Miltenyi Biotec (1 mentions)
Band pass filter, by IDEX Corporation (1 mentions)
3 protocols using isoflurane vaporizer
1
6-OHDA Injection for Parkinson's Model
2
Fundus Imaging of Dilated Mice
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Fundus examination was performed as previously described [42 (link)], using a Micron IV fundus camera (Phoenix Research Laboratories, Pleasanton, CA, USA), with the exception that 1% cyclopentolate or 1% atropine was used as the dilating agent, and mice were anesthetized with isoflurane (isoflurane vaporizer from Kent Scientific, Torrington, CT, USA) for the duration of imaging.
3
Two-Photon Imaging of GCaMP6m in Anesthetized Mice
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For two-photon imaging we used a TriM Scope II (LaVision BioTec GmbH, Germany) microscope and a 25x 4mm-working-distance, water-immersion objective (numerical aperture = 1, model XLPLN25XSVMP2, Olympus). Imaging was performed after ceasing of surgery-induced inflammation response, which in mice takes four weeks 29 . For excitation of GCaMP6m at 920 nm we used a standard titanium sapphire (Ti:Sa) laser (~900 fs laser pulse width at 80 MHz; Coherent). Fluorescence was assessed using a bandpass filter (510/20 nm; Semrock). For imaging under anesthesia, the rats were anesthetized with 1% isoflurane in oxygen using an isoflurane vaporizer (Kent Scientific, USA) and fixed with the metal bar to a holder in order to allow stable imaging.
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