The hair and serum samples were analyzed using the C-peptide kit from Roche Diagnostics GmbH in Germany and through electrochemiluminescence immunoassay (ECLISA) technology, the COBAS E411 analyzer (Hitachi High-Technologies Corporation). The detection limit of the C-peptide kit was: 0.003–13.3 nmol/L (0.010–40.0 ng/mL) and PreciControl Multimarker used as a control. The C-peptide level results measured in ng/mL were converted to mcg/dl and were then calculated to measure the C-peptide level in terms of picogram per milligram of hair sample. HbA1c and FPG were analyzed by using Absorbance Photometry assay by COBAS C111 HITACHI.
Cobas e411 analyzer
Manufactured by Hitachi
Sourced in Germany, Japan
The COBAS E411 analyzer is a compact, fully automated immunoassay analyzer designed for clinical laboratories. It performs a wide range of immunoassay tests on samples, including tests for hormones, tumor markers, and infectious diseases. The COBAS E411 offers efficient and reliable test results to support clinical decision-making.
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6 protocols using cobas e411 analyzer
1
Determining C-Peptide Levels in Hair and Serum
2
Blood Biomarker Analysis in Saudi Arabia
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All blood analyses were carried out at the King Abdulaziz Hospital, Jeddah, Saudi Arabia, and Al-Muzaylef General Hospital, Al-Muzaylef, Saudi Arabia.
The differential and complete CBC for all blood samples was carried out on a Sysmex XT 2000i Automated Hematology Analyzer (Sysmex, Kobe, Japan) using the Sysmex reagents (Sysmex, Norderstedt, Germany). A CRP Latex Test Kit (Crescent Diagnostics, Jeddah, Saudi Arabia) was used to determine the concentrations of CRP, as per the manufacturer’s instructions. The determination of the lipid profile for the subjects was carried out on a Beckman UniCel DxC 600 Chemistry Analyzer (Beckman Coulter, Brea, California), using chemicals from the same company. Vitamin D, T3, and T4 concentrations were determined on a Cobas e 411 Analyzer (Hitachi High-Technologies, Tokyo, Japan) using the Roche Diagnostic reagents (Mannheim, Germany).
The differential and complete CBC for all blood samples was carried out on a Sysmex XT 2000i Automated Hematology Analyzer (Sysmex, Kobe, Japan) using the Sysmex reagents (Sysmex, Norderstedt, Germany). A CRP Latex Test Kit (Crescent Diagnostics, Jeddah, Saudi Arabia) was used to determine the concentrations of CRP, as per the manufacturer’s instructions. The determination of the lipid profile for the subjects was carried out on a Beckman UniCel DxC 600 Chemistry Analyzer (Beckman Coulter, Brea, California), using chemicals from the same company. Vitamin D, T3, and T4 concentrations were determined on a Cobas e 411 Analyzer (Hitachi High-Technologies, Tokyo, Japan) using the Roche Diagnostic reagents (Mannheim, Germany).
3
Inflammatory Markers in PMX-DHP Treatment
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Serum inflammatory markers of white blood cells (WBCs), c-reactive protein (CRP), interleukin (IL)-6, and the ratio of partial pressure arterial oxygen to fraction of inspired oxygen (P/F ratio) were measured before and 48 hours after PMX-DHP treatment. IL-6 level was measured by electrochemiluminescence immunoassay using the Cobas e411 analyzer (Hitachi High-Technologies, Tokyo, Japan). We compared the results before and 48 hours after PMX-DHP treatment.
4
SHBG Serum Measurement in NHANES
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The laboratory method for detecting serum SHBG in NHANES was described in detail on website (https://wwwn.cdc.gov/Nchs/Nhanes/2013-2014/TST_H.htm https://wwwn.cdc.gov/nchs/data/nhanes/2013-2014/labmethods/TST_H_MET_Sex_Hormone_Binding_Globulin.pdf
5
Serum Biomarkers and Urinary Iodine Evaluation
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Venous blood was drawn from all the study participants, in the morning, after overnight fasting (>8 h). After 30 min of incubation at room temperature, the blood samples were centrifuged for 15 min (2500 rpm). Then, serum samples were frozen and stored vertically at −70 °C for further analyses.
TSH, free thyroxine (fT4), TPOAb and TGAb were measured in serum samples using an electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany) on a Cobas e411 Analyzer (Hitachi, Tokyo, Japan), in the Scientific Laboratory at the Department of Internal Medicine and Endocrinology, Medical University of Warsaw. Serum TPOAb and TGAb >34 IU/mL and >115 IU/mL respectively, were considered as “positive”.
Moreover, each study participant brought a 100 mL sample of midstream morning urine for the measurement of urinary iodine and creatinine. Urine iodine concentrations were estimated by the catalytic arsenium-cerium method based on Sandell–Kolthoff reaction [15 (link)]. Urinary creatinine was measured by colorimetric Jaffe method [16 (link)].
TSH, free thyroxine (fT4), TPOAb and TGAb were measured in serum samples using an electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany) on a Cobas e411 Analyzer (Hitachi, Tokyo, Japan), in the Scientific Laboratory at the Department of Internal Medicine and Endocrinology, Medical University of Warsaw. Serum TPOAb and TGAb >34 IU/mL and >115 IU/mL respectively, were considered as “positive”.
Moreover, each study participant brought a 100 mL sample of midstream morning urine for the measurement of urinary iodine and creatinine. Urine iodine concentrations were estimated by the catalytic arsenium-cerium method based on Sandell–Kolthoff reaction [15 (link)]. Urinary creatinine was measured by colorimetric Jaffe method [16 (link)].
6
Biomarkers of Cardiovascular Health
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Lipocalin-2 and endothelin-1 levels were measured using Quantikine ELISA immunoassay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol. Serum and urine levels of NT-pro-BNP were measured using an electrochemiluminescence immunoassay (ECLIA) on a Cobas e411 analyzer (Hitachi, Roche Diagnostics, Basel, Switzerland) according to the manufacturer's protocol. The detectable threshold for NT-proBNP levels was 5 pg/mL.
Complete blood count and hematological and urine parameters were taken routinely. Clinical and laboratory findings were retrospectively collected from patients' medical records, following standard measures for the protection of subjects' personal data.
Complete blood count and hematological and urine parameters were taken routinely. Clinical and laboratory findings were retrospectively collected from patients' medical records, following standard measures for the protection of subjects' personal data.
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