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Phalloidin tetramethyl rhodamine b isothiocyanate p1951

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Phalloidin-tetramethyl rhodamine B isothiocyanate (P1951) is a fluorescent dye used to label and visualize filamentous actin (F-actin) in cells. It binds specifically to F-actin, allowing researchers to study the distribution and dynamics of the actin cytoskeleton.

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Lab products found in correlation

Fluorescein isothiocyanate fitc conjugated anti α smooth muscle actin antibody f3777, by Merck Group (2 mentions) Fibronectin 610077, by BD (1 mentions) Horseradish peroxidase conjugated goat anti mouse rabbit secondary antibodies, by Promega (1 mentions) Pdgf bb, by Merck Group (1 mentions) Tlr4 sirna, by Thermo Fisher Scientific (1 mentions) Recombinant il 8, by Thermo Fisher Scientific (1 mentions) P gsk3β s9, by Cell Signaling Technology (1 mentions) Mouse anti gapdh monoclonal antibody, by ZSGB-BIO (1 mentions) Alpha tubulin, by Proteintech (1 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Tetramethyl rhodamine B isothiocyanate Phalloidin–tetramethyl–rhodamine Tetramethyl rhodamine B Rhodamine B isothiocyanate Rhodamine-phalloidin Rhodamine B P1951

4 protocols using phalloidin tetramethyl rhodamine b isothiocyanate p1951

1

Immunofluorescence and Cell Assays Protocol

Cited 2 times
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The following antibodies were used in this study: SHROOM2 (SAB2700199), Vinculin (V9131) and Phalloidin–Tetramethylrhodamine B isothiocyanate (P1951) were from Sigma; E-Cadherin (24E10), N-Cadherin (13116), MLC2 (3672), p-MLC2 (T18/S19) (3674), HA (3724) were from Cell Signaling Technology; ROCK1 (ab58305), CK5 (ab52635), CK10 (ab76318), Desmoglein1 (ab12077) and RAC1 (ab155938) were from abcam; p-MYPT (ABS45) was from Millipore and Fibronectin (610077) was from BD biosciences. Horseradish peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies were purchased from Promega.
Immunofluorescence microscopy, cell proliferation, colony formation, soft agar assays were performed as described previously8 (link),42 (link).
Yuan J., Chen L., Xiao J., Qi X.K., Zhang J., Li X., Wang Z., Lian Y.F., Xiang T., Zhang Y., Chen M.Y., Bei J.X., Zeng Y.X, & Feng L. (2019). SHROOM2 inhibits tumor metastasis through RhoA–ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death & Disease, 10(2), 58.
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2

Molecular Mechanisms of HSP60-Mediated Signaling

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Recombinant HSP60 was purchased from Assay Designs (Enzo Life Sciences, Switzerland). The HSP60 polyclonal antibody (15282-1-AP) was obtained from Proteintech (Proteintech Group, Inc., USA). ERK (BS1112), phospho-ERK (BS5016), and CD31 antibody (BS1574) were obtained from Bioworld Technology (St Paul, MN, USA). The ERK inhibitor U0126 and actin antibody were purchased from Cell Signaling Technology (USA). The TLR4 siRNA was obtained from Thermo (USA). PDGF-BB, IL-8, fluorescein isothiocyanate (FITC)-conjugated anti-α-smooth muscle actin antibody (F3777) and phalloidin-tetramethyl rhodamine B isothiocyanate (P1951) were obtained from Sigma-Aldrich (St Louis, MO, USA).
Zhao Y., Zhang C., Wei X., Li P., Cui Y., Qin Y., Wei X., Jin M., Kohama K, & Gao Y. (2015). Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation. Scientific Reports, 5, 15352.
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3

Probing PI3K/AKT Signaling Pathway

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Recombinant IL-8 was purchased from PeproTech, INC. Polyclonal antibodies against TMEM98 were purchased from Abcam. Alpha tubulin was purchased from Proteintech. Mouse anti-GAPDH monoclonal antibody was purchased from ZSGB-BIO. Monopoly antibodies against p-AKT (S473), p-AKT (T308), t-AKT, p-PI3K p85 (Y607), p-GSK3β (S9) and p-c-Raf (S259) were purchased from Cell Signaling Technology. The antibodies against p-ERK1/2, ERK and Cyclin D1 were purchased from Bioworld. The antibodies against of PI3K and p-PI3K were purchased from abcam. SC79 and BEZ235 were purchased from Selleck.cn. Fluorescein isothiocyanate (FITC)-conjugated anti-α-smooth muscle actin antibody (F3777) and phalloidin-tetramethyl rhodamine B isothiocyanate (P1951) were obtained from Sigma-Aldrich (St Louis, MO, USA).
Lv G., Zhu H., Li C., Wang J., Zhao D., Li S., Ma L., Sun G., Li F., Zhao Y, & Gao Y. (2017). Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis. Oncotarget, 8(50), 88043-88058.
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4

Lipopolysaccharide-Induced Cellular Responses

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Sterile dextrose (50% w/v in water) was from ROUX- OCEFA S.A. (Buenos Aires, Argentina). Lipopolysaccharide from Klebsiella pneumoniae (LPS, #L4268), Triton X-100 (octyl phenoxy polyethoxyethanol), Phalloidin–Tetramethylrhodamine B isothiocyanate (#P1951), and dimethyl sulfoxide (DMSO) were from Sigma-Aldrich (St. Louis, MO, USA). VU0359595 (PLD1 inhibitor) and VU0285655-1 (PLD2 inhibitor) were from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). DAPI (4′,6-diamidino-2-phenylindole dihydrochloride) was from Life Technologies Corporation (Grand Island, NY, USA). DCDCDHF (5(6)-carboxy-2′7′-dichlorodihydrofluorescein diacetate) was from Molecular Probes (Eugene, OR, USA). All other chemicals were of the highest purity available.
Bermúdez V., Tenconi P.E., Echevarría M.S., Asatrian A., Calandria J.M., Giusto N.M., Bazan N.G, & Mateos M.V. (2022). Targeting Phospholipase D Pharmacologically Prevents Phagocytic Function Loss of Retinal Pigment Epithelium Cells Exposed to High Glucose Levels. International Journal of Molecular Sciences, 23(19), 11823.
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