To assess strain growth, the optical density at 600 nm (OD600) was measured using a UV-2802PC; spectrophotometer (Unico, Shanghai, China). The concentrations of butyrate, butanol, and glucose in the fermentation samples were measured by HPLC using an Agilent 1260 system (Agilent Technologies, Santa Clara, CA, USA), equipped with an HPX-87H column (Bio-Rad Laboratories, Inc., Richmond, CA, USA) kept at 55 °C, with 5 mM H2SO4 at a flow rate of 0.5 mL/min as the mobile phase. The injection volume was 10 μL injection. For measurement of BB production, samples were taken from the solvent phase during fermentation, filtered, and immediately analyzed on a GCMS-QP2010 Ultra (Shimadzu, Japan) system equipped with a DB-5 ms column (30 m length, 0.25 mm inside diameter, 0.25 μm thickness, Agilent, USA). The flow rate of the helium carrier gas was 1 mL /min. The interface and ion source temperatures were set to 250 and 200 °C, respectively. The electron impact voltage was set to 70 eV. The m/z range was 35–500. The column temperature was initially set to 100 °C, after which it was increased to 250 °C at a rate of 20 °C/min, where it was held for 5 min.
Uv 2802pc spectrophotometer
Manufactured by Unico
Sourced in China
The UV-2802PC is a spectrophotometer that measures the absorption of ultraviolet and visible light by a sample. It is used to determine the concentration or identify the components of a substance.
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2 protocols using uv 2802pc spectrophotometer
1
Analytical Methods for Fermentation Monitoring
2
Analytical Techniques for Microbial Growth and Fermentation
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To assess strain growth, the optical density at 600 nm (OD 600 ) was measured using a UV-2802PC;
spectrophotometer (Unico, Shanghai, China). The concentrations of butyrate, butanol, and glucose in the fermentation samples were measured by HPLC using an Agilent 1260 system (Agilent Technologies, Santa Clara, CA, USA), equipped with an HPX-87H column (Bio-Rad Laboratories, Inc., Richmond, CA, USA) kept at 55°C, with 5 mM H 2 SO 4 at a ow rate of 0.5 mL/min as the mobile phase. The injection volume was 10 μL injection. For measurement of BB production, samples were taken from the solvent phase during fermentation, ltered, and immediately analyzed on a GCMS-QP2010 Ultra (Shimadzu, Japan) system equipped with a DB-5ms column (30 m length, 0.25 mm inside diameter, 0.25 μm thickness, Agilent, USA). The ow rate of the helium carrier gas was 1 ml/min. The interface and ion source temperatures were set to 250°C and 200°C, respectively. The electron impact voltage was set to 70 eV. The m/z range was 35-500. The column temperature was initially set to 100°C, after which it was increased to 250°C at a rate of 20°C/min, where it was held for 5 min.
spectrophotometer (Unico, Shanghai, China). The concentrations of butyrate, butanol, and glucose in the fermentation samples were measured by HPLC using an Agilent 1260 system (Agilent Technologies, Santa Clara, CA, USA), equipped with an HPX-87H column (Bio-Rad Laboratories, Inc., Richmond, CA, USA) kept at 55°C, with 5 mM H 2 SO 4 at a ow rate of 0.5 mL/min as the mobile phase. The injection volume was 10 μL injection. For measurement of BB production, samples were taken from the solvent phase during fermentation, ltered, and immediately analyzed on a GCMS-QP2010 Ultra (Shimadzu, Japan) system equipped with a DB-5ms column (30 m length, 0.25 mm inside diameter, 0.25 μm thickness, Agilent, USA). The ow rate of the helium carrier gas was 1 ml/min. The interface and ion source temperatures were set to 250°C and 200°C, respectively. The electron impact voltage was set to 70 eV. The m/z range was 35-500. The column temperature was initially set to 100°C, after which it was increased to 250°C at a rate of 20°C/min, where it was held for 5 min.
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