Lkc utas bigshot unit

Pupils of dark-adapted mice were dilated with Cyclogyl 1% (Alcon Pharmaceuticals, Fribourg, Switzerland) and Neosynephrine 5% (Ursapharm Schweiz GmbH, Roggwil, Switzerland). Mice were anesthetized by a subcutaneous injection of ketamine (85 mg/kg, Parke-Davis, Berlin, Germany) and xylazine (4 mg/kg, Bayer AG, Leverkusen, Germany). A drop of atropin 0.5% (Thea Pharma, Schaffhausen, Switzerland) was applied to each cornea just prior to placing gold ring electrodes onto each cornea. Recordings were done with an LKC UTAS Bigshot unit (LKC Technologies, Inc. Gaithersburg, MD, USA) using flash intensities from −50 db (0.000025 cd*s/m²) to 15 db (79 cd*s/m²) for scotopic and from −10 db (25 cd*s/m²) to 25 db (790 cd*s/m²) for photopic responses. Before photopic responses were recorded, mice were light-adapted for 5 min. Ten recordings were averaged per light intensity.
For fundus imaging and OCT scans, pupils were dilated and mice anesthetized as described above. A drop of 2% methocel (OmniVision AG, Neuhausen, Switzerland) was applied to keep eyes moist. Fundus images and OCT scans were acquired using the Micron IV system (Phoenix Research Labs, Pleasanton, CA, USA) as described [51 (link)].

Mice were dark-adapted overnight and pupils were dilated under dim red light with 1% cyclogyl (Alcon Switzerland SA, Rotkreuz, Switzerland) and 5% neosynephrin-POS (Ursapharm Schweiz GmbH, Roggwil, Switzerland) 30 minutes prior to recording. Mice were anesthetized with a subcutaneous injection of ketamine (85 mg/kg, Pfizer PFE Switzerland GmbH, Zurich, Switzerland) and xylazine (Rompun 2%, 4mg/kg, Bayer, Leverkusen, Germany). To keep the cornea moist, a drop of mydriaticum dispersa (Omnivision AG, Neuhausen, Switzerland) was applied to each eye. Low background illumination for 5 min was used for light adaptation. Electroretinograms were recorded simultaneously from both eyes with an LKC UTAS Bigshot unit (LKC Technologies, Inc. Gaithersburg, MD, USA) as described [32 (link)]. Flashes of 8 different light intensities ranging from -10 to 25 dB (0.25–790.5694 cd*s/m²) were applied under photopic conditions. 10 responses were averaged per light intensity. Traces from n≥4 mice were averaged for each light intensity.