Balb c mice

Both the murine retinal photoreceptor cell line 661W and Vero cells were obtained from Osaka Bioscience Institute. Both 661W and Vero cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 with l-glutamine and phenol red (661W) (Fujifilm Wako Pure Chemical Corporation) or high-glucose DMEM with l-glutamine, phenol red, HEPES, and sodium pyruvate (Vero) (Fujifilm Wako Pure Chemical Corporation), supplemented with 10% heat-inactivated foetal bovine serum (FBS) (Thermo Fisher Scientific) in a humidified incubator with 5% CO₂. Retinal microvascular endothelial cells of BALB/c mice were obtained from Cell Biologics and were cultured in complete mouse endothelial cell medium/w Kit (Cell Biologics) supplemented with 10% heat inactivated FBS (Thermo Fisher Scientific) in a humidified incubator with 5% CO₂.

The OKF6/TERT-2 oral epithelial cell line was provided by J. Rheinwald (Dana-Farber/Harvard Cancer Center, Boston, MA) and cultured as outlined previously (14 (link), 17 (link)). Primary oral mucosal epithelial cells from humans and BALB/c mice were obtained from Cell Biologics, Inc., and grown following the manufacturer’s instructions. NIH/3T3 cells that expressed human EGFR and HER2 were provided by Nadege Gaborit (Institut de Researche en Cancérologie de Montpellier, France) and grown as described previously (48 (link)). The C. albicans wild-type strain SC5314 and the als1Δ/Δ, als3Δ/Δ, and ece1Δ/Δ mutants (16 (link)) were grown in yeast extract-peptone-dextrose (YPD) broth in a shaking incubator at 30°C for 18 h, after which the cells were pelleted by centrifugation and washed twice with phosphate-buffered saline (PBS). Yeast cells were suspended in PBS, diluted, and counted with a hemacytometer.