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2 protocols using autopure wr 600g

1

Boronic Acid Derivatization Synthesis

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4-Carboxy-3-fluorophenylboronic acid, pinacol, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM), tetrahydrofuran, hydrochloric acid, sodium hydroxide, β-cyclodextrin, γ-cyclodextrin, fructose, glucose, galactose, phosphoric acid, sodium chloride, and dimethyl sulfoxide were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). 1,4-Dioxane, chloroform, acetonitrile, and dimethyl sulfoxide-d6 were purchased from Kanto Chemical, Co., Inc. (Tokyo, Japan). 2-Aminoanthracene was purchased from Sigma-Aldrich Japan, Co., LLC (Tokyo, Japan). All organic solvents and reagents were commercially available with guaranteed grades and used without further purification. Water was doubly distilled and deionized using a Milli-Q water system (WG222, Yamato Scientific Co., Ltd., Tokyo, Japan, and Autopure WR-600G, Merck Millipore, MA, USA) before use.
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2

Synthesis of a Fluorescent Probe

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2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), Acetonitrile (Luminasol), sodium hydroxide, and sodium nitrate were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 7-Hydroxycoumarin-3-carboxylic acid, 2,2′-dipicolylamine, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM), and formaldehyde (37% aqueous solution) were purchased from Tokyo Chemical Industry, Co., Ltd. (Tokyo, Japan). Acetonitrile, chloroform, and chloroform-d were purchased from Kanto Chemical, Co., Inc. (Tokyo, Japan). Sodium dihydrogen phosphate was purchased from Sigma-Aldrich Japan, Co., LLC. (Tokyo, Japan).
All other organic solvents and reagents were commercially available with guaranteed grades and used without further purification. Water was doubly distilled and deionized by a Milli-Q water system (WG222, Yamato Scientific Co., Ltd., Tokyo, Japan and Autopure WR-600G, Merck Millipore, MA, USA) before use.
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