Pe conjugated cd56

Manufactured by BioLegend

PE conjugated CD56 is a fluorescent-labeled antibody that recognizes the CD56 surface antigen. CD56 is a neural cell adhesion molecule expressed on natural killer cells, a subset of T cells, and other cell types. This product can be used for the identification and enumeration of CD56-positive cells in flow cytometry applications.

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Lab products found in correlation

Lysing solution, by BD (1 mentions) Fitc conjugated cd3, by BioLegend (1 mentions) Percp conjugated cd3, by BioLegend (1 mentions) Perm wash solution, by BD (1 mentions)

2 protocols using pe conjugated cd56

Degranulation Assay for NK Cells

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Analysis of cell surface–mobilized CD107a (degranulation assay) has been previously described [39 (link)]. Briefly, NK cells (50,000) were incubated either alone or with 721.221 target cells (50,000 cells), APC conjugated CD107a (BioLegend) and PE conjugated CD56 (BioLegend), for 2 hours in 37°C. Following incubation, CD107a expression was analyzed using flow cytometry.

Ophir Y., Duev-Cohen A., Yamin R., Tsukerman P., Bauman Y., Gamliel M, & Mandelboim O. (2016). PILRα binds an unknown receptor expressed primarily on CD56bright and decidual-NK cells and activates NK cell functions. Oncotarget, 7(27), 40953-40964.

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Multiparametric Immunophenotyping of Immune Cells

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Fluorescent‐conjugated anti‐human antibodies including fluorescein isothiocyanate (FITC)‐conjugated CD16 (clone: B73.1), phycoerythrin (PE)‐conjugated CD56 (5.1H11), Peridinin‐chlorophyll‐protein complex (PerCP)‐conjugated NKp44 (clone: P44‐8), Allophycocyanin (APC)‐conjugated CD3 (clone: OKT3), FITC‐conjugated CD3 (clone: OKT3), PerCP‐conjugated CD3 (clone: HIT3a), PerCp‐Cy5 conjugated CXCR3 (clone: G025H7), PerCp‐Cy5‐conjugated NKG2D (clone: 1D11), APC‐conjugated CD11b (clone: ICRF44), PerCP‐Cy5.5–conjugated CD27 (clone: M‐T271), PerCP‐Cy5.5‐conjugated perforin (clone: B‐D48), Alexa Fluor® 647‐conjugated granzyme B (clone: GB11), PerCP‐Cy5.5‐conjugated CD96 (clone: NK92.39) and their cognate isotype controls (all from Biolegend) as well as PerCP‐Cy5.5‐conjugated NKG2C (clone: 134591, R&D system) antibodies were used for immunophenotyping. Lysing solution (BD Biosciences) was used for removing the red blood cells. For washing, fixing, and cell permeabilizing, 1X phosphate buffer saline (PBS), 1% paraformaldehyde solution (Sigma), and 1X perm/wash solution (BD Biosciences) were used, respectively.

Darvishvand R., Rezaeifard S., Kiani R., Tahmasebi S., Faghih Z, & Erfani N. (2024). Natural killer cell subsets and their functional molecules in peripheral blood of the patients with breast cancer. Immunity, Inflammation and Disease, 12(4), e1255.

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