Fastlab fdg cassettes

¹⁸F-FDG was prepared using standard FASTlab FDG cassettes (GE Healthcare, UK) or TRACERlab MX kits (ROTEM, Israel) and formulated in phosphate buffer solution. ¹⁸F-FLT was prepared using a standard FASTlab FLT Cassette (GE Healthcare, UK) and was formulated in 9% ethanol in water. Radiochemical purity was >99% for both ¹⁸F-FDG and ¹⁸F-FLT.

[⁶⁸Ga]Pentixafor was synthesized using a fully automated Scintomics GRP+ module. The eluate of a ⁶⁸Ge/⁶⁸Ga-generator (GalliaPharm, EZAG, Germany) was concentrated using a Chromafix PS-H⁺ and heated with a solution of 40 µg Pentixafor (unlabeled precursor; GMP quality) in 1.5 mL of HEPES buffer (N-(2-hydroxyethyl)piperazine-N´-(2-ethanesulfonic acid), 1.5 M at 125°C for six minutes. Unbound and colloidal gallium-68 was removed using a SepPak Light C18 cartridge (Waters). The purified product was formulated with PBS buffer. The chemical and radiochemical purity of [⁶⁸Ga]Pentixafor were analyzed by high performance liquid chromatography (HPLC) using an analytic Chromolith® Performance, RP-18e, 100-4.6 mm from Merck (Germany). Free ⁶⁸Ga³⁺-ions and ⁶⁸Ga-colloid were detected using radio-thin-layer chromatography (radio-TLC).
The glucose analogue [¹⁸F]FDG was synthesized using FASTlab FDG cassettes with a phosphate buffer formulation and a GE FASTlab platform (GE Healthcare).