F. koreana Nakai branches and leaves were extracted with methanol and concentrated. Fruit extract was provided by Korea National Research Resource Bank. The extracts were dissolved with dimethyl sulfoxide (DMSO). Dulbecco’s modified Eagle medium (DMEM), FBS, Dulbecco’s PBS, α-minimum essential medium (α-MEM), and antibiotics were purchased from Gibco BRL (Grand Island, NY, USA). Recombinant mouse soluble RANKL (sRANKL) and macrophage-colony stimulating factor (M-CSF) were obtained from R&D Systems (Minneapolis, MN, USA). Histopaque-1083, MTT, DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Recombinant mouse soluble rankl srankl
Manufactured by R&D Systems
Sourced in United States
Recombinant mouse soluble RANKL (sRANKL) is a laboratory product manufactured by R&D Systems. It is a recombinant protein that represents the extracellular domain of the mouse RANKL (Receptor Activator of NF-κB Ligand) protein.
Automatically generated - may contain errors
Lab products found in correlation
Histopaque 1083, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Dulbecco s pbs, by Thermo Fisher Scientific (2 mentions)
Macrophage colony stimulating factor m csf, by R&D Systems (2 mentions)
Antibiotic antimycotic mixture, by Thermo Fisher Scientific (2 mentions)
α minimum essential medium α mem, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions)
Liensinine, by ChemFaces (1 mentions)
Nuciferine, by ChemFaces (1 mentions)
M csf, by R&D Systems (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
17β estradiol e2, by Merck Group (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
Alpha minimum essential medium α mem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
3 protocols using recombinant mouse soluble rankl srankl
1
Extraction and Characterization of F. koreana Bioactives
2
Nuciferine and Liensinine Cytotoxicity Assay
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Liensinine and nuciferine (≥98% by HPLC) were purchased from ChemFaces (Wuhan, Hubei, China) and dissolved with dimethyl sulfoxide (DMSO) and ethanol, respectively. Dulbecco's modified Eagle medium (DMEM), minimum essential medium alpha (α-MEM), Dulbecco's phosphate-buffered saline (PBS), fetal bovine serum (FBS), and antibiotic-antimycotic mixture containing 100 U/ml penicillin and 100 μl/ml streptomycin were obtained from Gibco BRL (Grand Island, NY). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), histopaque-1083, and DMSO were purchased from Sigma-Aldrich (St. Louis, MO). Anti-human Bcl-2, procaspase-3, caspase-3, poly(ADP-ribose) polymerase (PARP), and RANKL antibodies were obtained from Cell Signaling Technology (Beverly, MA). Anti-human Bax and horseradish peroxidase- (HRP-) conjugated anti-rabbit second antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant mouse soluble RANKL (sRANKL) and M-CSF were purchased from R&D system (Minneapolis, MN). All reagents used in this study were of analytical grade.
3
Artemisinin-Mediated Cellular Assays
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Artemisinin was purchased from LKT Laboratories (St. Paul, MN), and ARTD was provided by Professor Mankil Jung [21] . Minimum essential medium-alpha (α-MEM), RPMI 1640 medium, DMEM/nutrient mixture F-12 (DMEM/F-12) without phenol red, Dulbecco's PBS, HBSS, FBS, and antibiotic-antimycotic mixture were purchased from Gibco BRL (Grand Island, NY). TGF-β1 was purchased from Peprotech (London, England). Histopaque-1083, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and 17β-estradiol (E2) were purchased from Sigma-Aldrich (St. Louis, MO). Recombinant mouse soluble RANKL (sRANKL) and macrophage-colony-stimulating factor (M-CSF) were purchased from R＆D Systems (Minneapolis, MN). All reagents used in this study were of analytical grade.
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