hUCM-MSCs were washed in cold phosphate-buffered saline (PBS) and resuspended in 5 times the packed cell volume of PBS. Freezing and thawing was repeated three times. Normalised protein content was analysed with a Human Cytokine Array (ARY005B; R&D Systems) according to the manufacturer’s instructions.
Human cytokine array ary005b
Manufactured by R&D Systems
The Human Cytokine Array (ARY005B) is a multiplex sandwich immunoassay that allows for the simultaneous detection and quantification of 105 different human cytokines, chemokines, and other immune-related proteins in a single experiment. The array utilizes capture and detection antibodies to capture and measure the levels of these analytes in a variety of biological samples, such as cell culture supernatants, tissue lysates, and body fluids.
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Lab products found in correlation
2 protocols using human cytokine array ary005b
1
Cytokine profiling of hUCM-MSCs
2
Profiling Secreted Proteins in Cell Cultures
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The presence of 36 proteins in cell-free supernatants was determined using an R&D Systems human cytokine array (ARY005B) according to the manufacturer’s instructions.
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