Vero cells, derived from the kidneys of female green monkeys, were obtained from World Health Organization (WHO) (WHO, RCB 10‐87). Vero E6 was purchased from American Type Culture Collection (VERO C1008, Vero 76, clone E6, Vero E6). NutriVero FLEX‐20 and NutriVero Flex‐10 are serum‐free, animal component‐free medium. FLEX‐20 was specially designed for serum‐free adapter WHO, RCB 10‐87 Vero cells, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), nonessential amino acids (NEAAs), l ‐alanine‐l ‐glutamine, penicillin/streptomycin (pen/strep) antibiotics, recombinant trypsin‐EDTA solution, trypan blue solution, and phosphate‐buffered saline (PBS) were purchased from Biological Industries, Israel (05‐069‐1A, 05‐068‐1A, 01‐055‐1A, 04‐121‐1A, 01‐340‐1B, 03‐022‐1B, 03‐031‐1C, 03‐079‐1A, 03‐102‐1B, and 02‐020‐1A, respectively). OptiPRO SFM was purchased from Gibco (12309‐019). Fibra‐Cel carriers were purchased from Eppendorf (New Brunswick Scientific; M1292‐9984). Alamar blue was purchased from Promega (CellTiter‐blue, G808). Calcein AM was purchased from Sigma‐Aldrich (C1359). Tragacanth was purchased from Merck (G1128).
L alanine l glutamine
Manufactured by Sartorius
Sourced in Israel
L-alanine-L-glutamine is a dipeptide compound composed of the amino acids alanine and glutamine. It serves as a precursor for the synthesis of various biomolecules and is commonly used in cell culture and biomedical research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Sartorius (2 mentions)
Tragacanth, by Merck Group (1 mentions)
Optipro sfm, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Sartorius (1 mentions)
Recombinant trypsin edta solution, by Sartorius (1 mentions)
Trypan blue solution, by Sartorius (1 mentions)
Calcein am, by Merck Group (1 mentions)
Celltiter blue, by Promega (1 mentions)
Vero e6, by American Type Culture Collection (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Non essential amino acids (neaa), by Sartorius (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Millex hv, by Merck Group (1 mentions)
2 protocols using l alanine l glutamine
1
Vero Cell Culture for SARS-CoV-2 Research
2
Lentiviral Production of SARS-CoV-2 Proteins
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HEK293T cells (ATCC, Manassas, VA) were seeded in 10 cm cell culture plates at a density of 4 × 106 cells/plate. The cells were maintained in 293T medium composed of DMEM high glucose (4.5 g/l; Merck, Rahway, NJ) supplemented with 10 % fetal bovine serum (FBS; Biological Industries), 1× NEAA (Biological Industries), and 2 mM L-alanine–L-glutamine (Biological Industries, Israel). Lentiviral stocks, pseudo-typed with VSV-G, were produced in HEK293T cells as previously described (Kutner et al., 2009 (link)). Briefly, each of the pLVX plasmids containing the SARS-CoV-2 genes or EGFP for control were cotransfected with third-generation lentivirus helper plasmids at equimolar ratio; 48 hr later, the lentivirus-containing medium was collected and supernatant was clarified by centrifugation (500 g, 5 min) and filtration (0.45 µm, Millex-HV, Merck Millipore, Burlington, MA). All virus stocks were aliquoted and stored at –80 °C until thawed for subsequent use.
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