Leucine dehydrogenase (LDH) from Bacillus cereus was in our laboratory. Plasmid pET-28a (+) and Escherichia coli BL21 (DE3) were procured from Novagen (Nanjing, China) and used as the gene expression vector and host, respectively. Kanamycin, isopropyl-β-D-thiogalactopyranoside (IPTG), 1-fluoro-2,4-dinitrophenyl-5-L-alanineamide (FDAA), NAD+, and NADH were obtained from TCI (Shanghai, China). α-keto acids (4-methyl-2-oxopentanoic acid, 3-methyl-2-oxobutanoic acid, 2-oxobutanoic acid, 3,3-dimethyl-2-oxobutanoic acid, 2-oxopropanoic acid, 2-oxo-3-phenylpropanoic acid) and α-amino acids (L-leucine, L-valine, L-2-aminobutyric acid, L-tert-leucine, L-alanine, L-phenylalanine) were purchased from J&K Chemical (Beijing, China). All other chemicals were purchased from commercial sources, at least reagent grade, and were used without further purification.
L valine
Manufactured by J&K Scientific
Sourced in China
L-valine is an amino acid that is essential for human and animal nutrition. It is a colorless, crystalline solid with the chemical formula C5H11NO2.
Automatically generated - may contain errors
Lab products found in correlation
L phenylalanine, by J&K Scientific (1 mentions)
L leucine, by J&K Scientific (1 mentions)
Gemini 300, by Zeiss (1 mentions)
Cell free amino acid mixture 15n, by Merck Group (1 mentions)
Microflex lrf mass spectrometer, by Bruker (1 mentions)
L serine, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Cyclohexane, by Merck Group (1 mentions)
L aspartic acid, by Merck Group (1 mentions)
Methanol, by Sinopharm (1 mentions)
Acetonitrile, by Sinopharm (1 mentions)
Ethanol, by Sinopharm (1 mentions)
Haucl4 4h2o, by Sinopharm (1 mentions)
2 protocols using l valine
1
Bacillus cereus Leucine Dehydrogenase Purification
2
Synthesis and Characterization of Gold Nanoparticles
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Trifluoroacetic acid (TFA, 99%), α-cyano-4-hydroxycinnamic acid (CHCA, 98%), cyclohexane (99.5%), l -serine (99%), l -aspartic acid (98%), cell free amino acid mixture-15N (98%, 767972-1 EA) and 1H,1H,2H,2H-perfluorodecanethiol (PFDT, 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HAuCl4·4H2O (99.9%), acetonitrile (99.9%), ethanol (99.7%) and methanol (99.8%) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). l -arginine (99%), l -valine (99%) and sodium citrate tribasic dihydrate (99%) were purchased from J&K Scientific (Beijing, China). Novec™ 7500 Engineered Fluid (HFE-7500) was purchased from Minnesota Mining and Manufacturing Corporation (Maplewood, MN, USA). Deionized (DI) water (18.2 MΩ cm) used in all experiments was obtained from a Milli-Q system (Millipore, Bedford, MA, USA). SEM images were collected on a Zeiss Gemini 300 scanning electronic microscopy (Carl Zeiss AG, Oberkochen, Germany). MALDI-TOF MS analysis was performed with a Bruker MicroFlex LRF mass spectrometer (Bruker, Bremen, Germany). Optical microscopic image was obtained with inverted microscope (Cewei Optoelectronic Technology Ltd, Shanghai, China).
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