Serum separating tubes

Manufactured by Sarstedt

Sourced in Germany

Serum-separating tubes are a type of laboratory equipment used to separate serum from whole blood samples. They contain a gel-like substance that creates a barrier between the serum and cellular components during centrifugation, allowing for easy separation and collection of the serum portion.

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Lab products found in correlation

Opteia mouse ige elisa set, by BD (1 mentions)

3 protocols using serum separating tubes

Mouse Serum IgE Measurement

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Blood was collected in serum-separating tubes (Sarstedt and BD) and total serum IgE concentrations were measured using either the BD OptEIA Mouse IgE ELISA Set (BD) according to the manufacturer's instructions, or a multi-array (Meso Scale Discovery) utilizing the BD OptEIA Mouse IgE ELISA Set.

Wyss M., Brown K., Thomson C.A., Koegler M., Terra F., Fan V., Ronchi F., Bihan D., Lewis I., Geuking M.B, & McCoy K.D. (2020). Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE. Frontiers in Immunology, 10, 3107.

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Tissue Collection and Preservation Protocol

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At defined time points, mice were weighed and euthanized by CO₂, followed by cervical dislocation; blood was collected by cardiac puncture, and serum was collected using serum-separating tubes (#41.1378.005, Sarstedt), aliquoted, and stored at −80 °C. Urogenital apparatus and liver tissues were fixed in 10% buffered formalin and processed for paraffin embedding. Alternatively, mouse prostate lobes (anterior prostate, AP; dorsolateral prostate, DLP; ventral prostate, VP) were immediately dissected, weighed and flash-frozen in liquid nitrogen. Serum and tissues were consistently collected during the same periods to minimise inter-samples and circadian rhythm variability.

Labbé D.P., Zadra G., Yang M., Reyes J.M., Lin C.Y., Cacciatore S., Ebot E.M., Creech A.L., Giunchi F., Fiorentino M., Elfandy H., Syamala S., Karoly E.D., Alshalalfa M., Erho N., Ross A., Schaeffer E.M., Gibb E.A., Takhar M., Den R.B., Lehrer J., Karnes R.J., Freedland S.J., Davicioni E., Spratt D.E., Ellis L., Jaffe J.D., DʼAmico A.V., Kantoff P.W., Bradner J.E., Mucci L.A., Chavarro J.E., Loda M, & Brown M. (2019). High-fat diet fuels prostate cancer progression by rewiring the metabolome and amplifying the MYC program. Nature Communications, 10, 4358.

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Seroprevalence of Bartonella Infections

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Blood samples (9 mL) from blood donors (n = 65) and patients (n = 40) were collected in plastic EDTA tubes (Sarstedt, Germany). At the same time, serum samples (9 mL) were collected into serum-separating tubes (Sarstedt, Germany) to examine the presence of antibodies against B. henselae and B. quintana. Aseptic technique was used; an experienced nurse performed venipuncture. Samples were transported to the laboratory within 2 h and processed immediately. The remaining blood samples, in EDTA tubes, and serum, distributed in 1.5-mL Eppendorf tubes, were stored at -70 °C for further use.

Łysakowska M.E., Brzezińska O., Szybka M., Konieczka M., Moskwa S., Brauncajs M., Makowska J., Pastuszak-Lewandoska D, & Grzegorczyk J. (2019). The seroprevalence of Bartonella spp. in the blood of patients with musculoskeletal complaints and blood donors, Poland: a pilot study. Clinical rheumatology, 38(10).

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