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3 protocols using antibodies against phospho stat1

1

Immune Cell Signaling Pathway Analysis

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GA (Copolymer I (P1152)), Minimum Essential Medium Eagle (MEM) and Salmonella typhimurium Lipopolysaccharide (LPS) were obtained from Sigma-Aldrich (St. Louis, MO). Dulbecco’s modified eagle’s medium (DMDM) and Fetal Bovine Serum (FBS) were purchased from HyClone (Logan, UT). Rat interferon (IFN) γ was purchased from Calbiochem (La Jolla, CA). Antibodies against phospho-STAT1, total STAT1, phospho-STAT3 and total STAT3 were purchased from Cell Signaling Technology (Danvers, MA). Tubulin antibody was purchased from Sigma-Aldrich (St. Louis, MO).
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2

Innate Immune Signaling Pathway Components

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Poly(dA:dT), poly(dG:dC), and LyoVec were purchased from InvivoGen (San Diego, CA, USA). All the culture reagents were purchased from Gibco (Grand Island, NY, USA). The antibodies against phospho-STAT1, STAT1, phospho-IRF7, IRF7, IRF3, ISG15, ISG56, Viperin, GBP5, cGAS, GAPDH and horseradish peroxidase conjugated second antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibodies against phospho-IRF3, MxB and OAS2 were obtained from Abcam (Cambridge, MA, USA). The antibodies against IFI16, ISGF3γp48 were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The antibody against BST-2 was obtained from the AIDS Research and Reference Reagent Program at the National Institutions of Health (NIH). The neutralizing anti-human IFNAR2 antibody was purchased from PBL assay science (Piscataway, NJ, USA). The neutralizing antibodies against human IL-28Rα and IL-10Rβ were purchased from R&D system Inc (Minneapolis, MN, USA).
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3

Antibody Panel for Macrophage Characterization

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The anti-F4/80 antibody was purchased from Bio-Rad (Hercules, CA, USA). The anti-iNOS antibody was purchased from BD Transduction Laboratories (San Jose, CA, USA), Thermo Scientific (Fremont, CA, USA) or Abcam (Cambridge Science Park, Cambridge, UK). CX3CR1 was obtained from Bioss (Woburn, Massachusetts, USA). CD68 was obtained from Abcam and CD206 from Bioss, BIO-RAD (Hercules, CA, California) or R&D Systems (Minneapolis, MN, USA). Antibodies against phospho-STAT1, STAT1, phospho-STAT3, STAT3 and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA).
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