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Ab54037

Manufactured by Abcam
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Sourced in United States

Ab54037 is a primary antibody that can be used for the detection of a specific target protein in various experimental techniques. This product is suitable for applications such as Western blotting, immunohistochemistry, and immunocytochemistry. The core function of this antibody is to specifically bind and detect the target protein in samples.

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Lab products found in correlation

Etomoxir, by Merck Group (1 mentions) Menadione, by Merck Group (1 mentions) Oligomycin, by Merck Group (1 mentions) Pf 06424439, by Merck Group (1 mentions) Ebselen, by Bio-Techne (1 mentions) Azd 3988, by Bio-Techne (1 mentions) Tempol, by Merck Group (1 mentions) Gfp 2956, by Cell Signaling Technology (1 mentions) Paraquat, by Merck Group (1 mentions) Atn 224, by Cayman Chemical (1 mentions) Antibody against erk2 c14, by Santa Cruz Biotechnology (1 mentions) Ml385, by Selleck Chemicals (1 mentions) T5326, by Merck Group (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (1 mentions) Antimycin a, by Merck Group (1 mentions) Rotenone, by Merck Group (1 mentions) Ferrostatin 1, by Merck Group (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) Ab46545, by Abcam (1 mentions) Flagellin, by InvivoGen (1 mentions)

3 protocols using ab54037

1

Mitochondrial Stress Response Modulators

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Compounds were used at the following concentrations unless otherwise noted: 50 μM AZD3988 (Tocris), 30 μM A922500 (Stratech), 50 μM AZD7687 (Stratech), 70 μM T863 (Sigma), 1 μM Oligomycin (Sigma), 0.5 μM FCCP (Sigma), 1 μM Antimycin-A (Sigma), 1 μM Rotenone (Sigma), 50 μM PF-06424439 (Sigma), 5 μM Ebselen (Tocris), 1 mM Tempol, 200 μM Paraquat (Sigma), Menadione (Sigma), 100 μM Etomoxir (Sigma), 2 μM Ferrostatin-1 (Sigma), 5 μM ML385 (Selleckchem), and 1 μM ATN-224 (Cayman Chemical). Antibodies against DGAT1 (ab54037) and 4-Hydroxynonenal (ab46545) were purchased from abcam. Antibodies against Vinculin (66305-1-Ig), Beta-Tubulin (10094-1-AP), PINK1 (23274-1-AP), Parkin (14060-1-AP), SOD1 (10269-1-AP), SOD2 (24127-1-AP), SESN2 (10795-1-AP) and GAPDH (60004-1-Ig) were purchased from Proteintech. Antibodies against phospho-AMPK (50081), phospho-RAPTOR (2083), Caspase-3 (9662), phospho-ERK (4370) and GFP (2956) were purchased from Cell Signalling. The antibody against gamma-tubulin (T5326) was purchased from Sigma. The antibody against ERK2 (C-14) was from Santa Cruz Biotechnology.
Wilcock D.J., Badrock A.P., Wong C.W., Owen R., Guerin M., Southam A.D., Johnston H., Telfer B.A., Fullwood P., Watson J., Ferguson H., Ferguson J., Lloyd G.R., Jankevics A., Dunn W.B., Wellbrock C., Lorigan P., Ceol C., Francavilla C., Smith M.P, & Hurlstone A.F. (2022). Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breached. Cell Reports, 39(12), 110995.
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2

Inflammasome Activation and Lipid Metabolism

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Lipopolysaccharide (LPS) (Escherichia coli; tlrl-pelps) and flagellin (Salmonella typhimurium; tlrl-stfla) were from Invivogen (San Diego, CA, USA). Adenosine triphosphate (ATP) (A2383), while nigericin (N7143), poly(dA:dT) (P0883), and PF-04620110 (PZ0207) were from Sigma-Aldrich (St. Louis, MO, USA). The following antibodies were used: polyclonal rabbit anti-caspase-1 for mouse caspase-1 (1:1,000) (SC-514; Santa Cruz Biotechnology, Dallas, TX, USA), polyclonal goat anti-IL-1β for mouse IL-1β (1:1,000) (AF-401-NA; R&D Systems, Minneapolis, MN, USA), polyclonal rabbit anti-ASC for mouse ASC (1:1,000) (ADI-905-173-100; Enzo Lifesciences, Farmingdale, NY, USA), polyclonal rabbit anti-DGAT1 for mouse DGAT1 (1:1,000) (ab54037; Abcam, Cambridge, MA, USA), and monoclonal mouse anti-β-actin (1:5,000) (A5316; Sigma-Aldrich).
Jo S.I., Bae J.H., Kim S.J., Lee J.M., Jeong J.H, & Moon J.S. (2019). PF-04620110, a Potent Antidiabetic Agent, Suppresses Fatty Acid-Induced NLRP3 Inflammasome Activation in Macrophages. Diabetes & Metabolism Journal, 43(5), 683-699.
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3

Western Blot Antibody Analysis

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Western blot analyses were carried out as described previously33 (link). The following antibodies were used: TGF-β1 (ab92486, Abcam), α-SMA (23779489, Millipore), collagen type I (ab21286, Abcam), ACL (ab40793, Abcam), HMGCR (ab174830, Abcam), DGAT1 (ab54037, Abcam), Gp91 (ab129068, Abcam), TNF-α (ab9739, Abcam), Caspase-3 (9662, Cell Signaling), PUMA (ab9643, Abcam), Cytochrome c (ab133504, Abcam), Drp1 (5391, Cell Signaling), and phospho-Drp1(Ser616) (4494, Cell Signaling).
Sun Y., Ge X., Li X., He J., Wei X., Du J., Sun J., Li X., Xun Z., Liu W., Zhang H., Wang Z.Y, & Li Y.C. (2020). High-fat diet promotes renal injury by inducing oxidative stress and mitochondrial dysfunction. Cell Death & Disease, 11(10), 914.
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