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Dh5α strain of e coli

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DH5α is a common strain of Escherichia coli (E. coli) bacteria. It is a laboratory-derived strain that is frequently used in molecular biology and genetic engineering applications. The DH5α strain is designed to be highly efficient for the transformation and propagation of plasmid DNA.

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Lab products found in correlation

Bl21 de3 strain of e coli, by Thermo Fisher Scientific (2 mentions)

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E. coli DH5α (180 citations) E. coli DH5α strain (36 citations) DH5α strain (2 citations)

2 protocols using dh5α strain of e coli

1

Purification of S.c. DNA Repair Complexes

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The expression plasmids of S.c. Rad24-RFC, 9-1-1 clamp and RPA were generated by standard molecular biology techniques, constructed using the DH5α strain of E. coli (Thermo Fisher Scientific), and are listed in the key resources table. Those three multiprotein complexes were purified from the BL21(DE3) strain of E. coli (Thermo Fisher Scientific). E. coli strains were cultured in LB media.
Zheng F., Georgescu R.E., Yao N.Y., O’Donnell M.E, & Li H. (2023). Structures of 9-1-1 DNA checkpoint clamp loading at gaps from start to finish and ramification on biology. Cell reports, 42(7), 112694.
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2

S.c. Rad24-RFC, 9-1-1 Clamp, and RPA Purification

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The expression plasmids of S.c. Rad24-RFC, 9-1-1 clamp and RPA were generated by standard molecular biology techniques, constructed using the DH5α strain of E. coli (Thermo Fisher Scientific), and are listed in the key resources table. Those three multiprotein complexes were purified from the BL21(DE3) strain of E. coli (Thermo Fisher Scientific). E. coli strains were cultured in LB media.
Zheng F., Georgescu R.E., Yao N.Y., O’Donnell M.E, & Li H. (2023). Structures of 9-1-1 DNA checkpoint clamp loading at gaps from start to finish and ramification to biology. bioRxiv.
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