Lysates from primary melanocytes were purchased from Sciencell Research Laboratories (Carlsbad, CA) and NCI-60 validated human melanoma cell lines obtained from the University of Hawaii Cancer Center included SK-Mel2, SK-Mel28, and MALME-3M. These cell lines were cultured in RPMI media with 10% fetal bovine serum and 1% Antibiotic-Antimycotic (all from GIBCO/Thermo Fisher). Primary antibodies for western blots included rabbit monoclonal anti-SELENOK (Epigemonics, Inc., custom antibody), anti-IP3R1 (Santa Cruz Biotechnology, sc-271197), anti-GAPDH (Santa Cruz Biotechnology, sc-47724), anti-Prom1 (Cell Signaling, 58605). Antibodies from Abcam included anti-TRP2 (ab74073), anti-calcineurin A and B (ab137335, ab154650), and anti-calmodulin (ab 105498, respectively). Western blot secondary antibodies were purchased from Li-Cor Technologies and immunofluorescence secondary Alexafluor595 antibody from Thermo. The transgene encoding full-length SELENOK in pcDNA3.1+ (Invitrogen) has been previously described [11 (link)].
Ab154650
Manufactured by Abcam
Sourced in United States
Ab154650 is a lab equipment product offered by Abcam. It is a device used for scientific research purposes. The core function of this product is to perform a specific task in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ab74073, by Abcam (1 mentions)
Alexafluor 595, by Thermo Fisher Scientific (1 mentions)
Ab137335, by Abcam (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti ip3r1, by Santa Cruz Biotechnology (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Pcdna3, by Thermo Fisher Scientific (1 mentions)
St040, by Beyotime (1 mentions)
2 protocols using ab154650
1
Evaluation of SELENOK in Melanoma Cells
2
Colorimetric Assay for Calcineurin
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Lyophilized CNAΔ316, CNB standard, CNB test sample (Beijing Normal University), p-nitrophenylphosphate (pNPP) (34047, Thermo Fisher Science, USA), bovine serum albumin (BSA) (ST023 Beyotime, Shanghai), DLdithiothreitol (DTT) (ST040, Beyotime, Shanghai), and anti-CNB polyclonal antibody (ab154650, Abcam, USA) were used.
Testing medium comprised 50 mmol L 1 Tris-HCl (pH 7.4), 0.2 mg mL 1 BSA, 20 mmol L 1 pNPP, 1 mmol L 1 DTT, 1 mmol L 1 MnCl 2 , and 1 mmol L 1 CaCl 2 . Microtiter plate reader (Spectrum plus 5, Molecular Device, USA) was used in the assays.
Testing medium comprised 50 mmol L 1 Tris-HCl (pH 7.4), 0.2 mg mL 1 BSA, 20 mmol L 1 pNPP, 1 mmol L 1 DTT, 1 mmol L 1 MnCl 2 , and 1 mmol L 1 CaCl 2 . Microtiter plate reader (Spectrum plus 5, Molecular Device, USA) was used in the assays.
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