The acoustically confined HGSs with surface-bound antibodies were treated for 2 h in a solution of sulfo-Cyanine3 (Cy3)-NHS ester (Lumiprobe Corp.) dissolved in a pH 8.0 buffer (potassium phosphate monobasic/sodium hydroxide, Fisher Scientific Co.) to 2 mg dye per 1 mL buffer. The reagent solution was introduced at 7 μL/min for 15 min and incubated at 23 °C for 2 h before removal using a 1 × PBS wash (7 μL/min for 15 min). Acoustic actuation was exclusively used during reagent introduction and the buffer wash as a precaution to minimize heating and/or potential damage due to ultrasound exposure during incubation.
Potassium phosphate monobasic sodium hydroxide
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Potassium phosphate monobasic/sodium hydroxide is a laboratory reagent used for buffer preparation. It is a mixture of potassium phosphate monobasic and sodium hydroxide, which can be used to create buffer solutions with a range of pH values. The specific function of this product is to provide a controllable pH environment for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sulfo cyanine3 cy3 nhs ester, by Lumiprobe (1 mentions)
Absolute ethanol, by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Magnesium stearate, by Merck Group (1 mentions)
Methocel, by Colorcon (1 mentions)
Anhydrous theophylline, by Tokyo Chemical Industry (1 mentions)
2 protocols using potassium phosphate monobasic sodium hydroxide
1
Fluorescent Labeling of Antibody-Coated Hydrogel Beads
2
Theophylline Tablet Formulation Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Methocel (HPMC K4M) was a kind gift from Colorcon (UK). Lactose monohydrate (FlowLac ® 100) was a kind gift from Meggle (Germany). Magnesium stearate obtained from Merck (Germany). Anhydrous theophylline was obtained from TCI Chemicals (Europe). Potassium chloride, hydrochloric acid, potassium phosphate monobasic sodium hydroxide, absolute ethanol were obtained from Fisher Scientific (UK). absolute ethanol (Fisher Scientific, UK) was used to produce the hydro-alcoholic solutions in 5-40 % v/v with either 0.1N HCl (pH 1.2) or phosphate buffer (pH 6.8). Native SG was extracted in our laboratory as previously reported [22] .
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