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2 protocols using 3 appa

1

Comprehensive Reagent Acquisition for Research

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GABA (Cat. A2129), CP 55940 (Cat. C1112) and carbachol (PHR1511) were purchased from Sigma-Aldrich (Shanghai, China). R-baclofen (Cat. 0796), SKF 97581 (Cat. 0379) and CGP54626 (Cat. 1088) were obtained from Tocris Biosciences (Shanghai, China). 3-APPA (Cat. ab120329) was purchased from Abcam (Shanghai, China). Win 55,212-2 (T4458), Bay 59-3074 (T3699), DAMGO (T4351), eletriptan HBr (T0216), pramipexole 2HCl monohydrate (T6951), brimonidine tartrate (T6422), tizanidine hydrochloride (T0290), oxymetazoline hydrochloride (T0252), xylazine (T7046), clonidine hydrochloride (T1247), and 1-oleoyl lysophosphatidic acid sodium (T21654) were purchased from TargetMol (Shanghai, China). PAF C-16 (74389-68-7) was purchased from Santa Cruz Biotechnology (Shanghai, China). CGP64213 was a gift from Prof. Fajun Nan (Shanghai Institute of Materials Medicine, China). Coelenterazine h (Cat. S2001) and furimazine (Cat. N1120) were purchased from Promega (Madison, WI, USA).
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2

GABA receptor binding assay

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Materials and Reagents GABA, CGP54626, and SKF97541 were purchased from Tocris Bioscience. Baclofen, 3-APPA, CGP35348, CGP7930, GS39783, and rac-BHFF were purchased from Abcam. Lipofectamine 2000 and Fluo-4 AM were from Thermo Fischer Scientific. SNAP-Lumi4-Tb, SNAP-Green, SNAP-Red, CoA-Lumi4-Tb, and Tag-Lite buffer can be purchased from Cisbio Bioassays. CoA-Alexa 488, CoA-647, and Sfp synthase were from New England Biolabs. Benzyl guanine (BG) derivative fluorophores BG-Green-LC and BG-Atto465, and CGP54626-Red were a gift from Cisbio Bioassays.
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