AST was performed using disk diffusion method (Kirby–Bauer) on Mueller–Hinton agar (Merck) plates according to the Clinical and Laboratory Standards Institute (CLSI) Guidelines.22 The tested antibiotic disks were colistin (10 μg), ciprofloxacin (5 μg), tetracycline (30 μg), imipenem (10 μg), ceftazidime (30 μg), azithromycin (15 μg) and amikacin (30 μg) (MAST Co., UK). The phenotype of Enterobacteriaceae was defined as MDR according to the International Expert proposal for Interim Standards Guidelines.6 (link)
Azithromycin
Manufactured by Mast Group
Sourced in United Kingdom
Azithromycin is a macrolide antibiotic commonly used in laboratory settings. It is a broad-spectrum antibiotic that inhibits bacterial protein synthesis, effectively targeting a variety of gram-positive and gram-negative pathogens.
Automatically generated - may contain errors
Lab products found in correlation
Amikacin, by Mast Group (2 mentions)
Imipenem, by Mast Group (2 mentions)
Tetracycline, by Mast Group (1 mentions)
Colistin, by Mast Group (1 mentions)
Mueller hinton agar (mha), by Merck Group (1 mentions)
Ceftazidime, by Mast Group (1 mentions)
Ciprofloxacin, by Mast Group (1 mentions)
Ceftriaxone, by Mast Group (1 mentions)
Chloramphenicol, by Mast Group (1 mentions)
Gentamycin, by Mast Group (1 mentions)
Tobramycin, by Mast Group (1 mentions)
Penicillin, by Mast Group (1 mentions)
3 protocols using azithromycin
1
Antibiotic Susceptibility Testing of Enterobacteriaceae
2
Antimicrobial Susceptibility of Lactobacillus
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Qualitative antibacterial susceptibility of the microorganisms was determined according to the standard disk diffusion (Kirby-Bauer) method (20 (link)). using the paper disk including (μg/disc): azithromycin (15 (link)); ceftriaxone (21 (link)); imipenem (10 (link)); amikacin (21 (link)); chloramphenicol (21 (link)); ceftazidim (21 (link)); tobramycin (10 (link)); gentamycin (10 (link)) andciprofloxacin (5 (link)) purchased from Mast Co (Liverpool, UK).
Microbial suspensions with 106 colony forming units (CFU/mL) of each Lactobacillus strain in NB were prepared on a De Mann Rogosa Sharpe agar plate. The plates were incubated at 37°C for 48 hours in anaerobic condition and examined for the inhibition zone diameter appearing around each antibiotic disc. A test was carried out thrice for each antibiotic agent . Inhibitory zone diameters were compared with the standards provided by the National Committee for Clinical Laboratory Standards (NCCLS) (19 ). Antibiotic sensitivity of P. aeruginosa was also determined by the same method using the Muller Hilton agar.
Microbial suspensions with 106 colony forming units (CFU/mL) of each Lactobacillus strain in NB were prepared on a De Mann Rogosa Sharpe agar plate. The plates were incubated at 37°C for 48 hours in anaerobic condition and examined for the inhibition zone diameter appearing around each antibiotic disc. A test was carried out thrice for each antibiotic agent . Inhibitory zone diameters were compared with the standards provided by the National Committee for Clinical Laboratory Standards (NCCLS) (19 ). Antibiotic sensitivity of P. aeruginosa was also determined by the same method using the Muller Hilton agar.
3
Antibiotic Resistance Profile of N. gonorrhoeae
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The resistance profile of the confirmed N. gonorrhoea for seven antibiotics (Mast Group Ltd, UK) – penicillin (PG: 10 units), azithromycin (ATH: 15 µg), ceftriaxone (CRO: 30 µg), ciprofloxacin (CIP: 5 µg), erythromycin (E: 15 µg), togamycin (TG: 10 µg) and tetracycline (T: 30 µg) – was evaluated using the disc diffusion method following the procedure and guidelines of the Clinical and Laboratory Standards Institute (CLSI) [21 ]. For azithromycin, the Centers for Disease Control and Prevention (CDC’s) breakpoint was used to interpret its results. Triplicate averages of the inhibition zones were used for this purpose. The ratio of antibiotics resisted by an isolate to the number of antibiotics tested on the isolate, multiplied by 100%, was used to indicate an antibiotic’s antimicrobial resistance percentage. Multiple AMR index was evaluated as a ratio of the number of antibiotics an isolate is resistant to the number of antibiotics evaluated on the isolate as previously described [19 (link)].
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