To prepare samples for cryo-EM study, lacey carbon grids (Cu, 300 mesh) were glow-discharged (45s at MED RF power level) in Harrick plasma cleaner. 4 mL of sample solution was applied on to the carbon side of EM grid, which was then blotted for 2.5 s at 95% humidity and plunge-frozen into Leica GP2 plunger (Queen Mary University of London). Grids were imaged on a Jeol JEM-2100 plus (Queen Mary University of London), equipped with Gatan OneView 16MP camera at accelerating voltage of 200 kV. Images were obtained using SerialEM software at low dose mode at 50k magnification, corresponding to 2.166A ˚with the defocus range set from -2.5 to -5 mm.
Oneview 16mp camera
Manufactured by Ametek
The OneView 16MP camera is a high-resolution imaging device designed for laboratory and scientific applications. It features a 16-megapixel sensor that captures detailed images. The camera's core function is to provide precise visual data for analysis and documentation purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using oneview 16mp camera
1
Cryo-EM Sample Preparation Protocol
2
TLNRD1-F-actin Interaction Visualization
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F-actin was diluted to 25 µM in polymerisation buffer. TLNRD1-FL was incubated with the Factin at a 1:1 ratio at room temperature for 1 hr. After incubation, samples were diluted down to 2 µM with polymerisation buffer. Samples were applied to 300 mesh carbon-coated copper grids for 30 seconds, excess solution removed and negatively stained for 1 minute with 2% (wt/vol) uranyl acetate. Excess stain was removed and grids washed under a stream of ddH2O. Grid samples were dried before imaging. Images were taken on a JEOL-1230 transmission electron microscope equipped with a Gatan One View 16 MP camera and an accelerating voltage of 80 kV.
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