Acetonitrile analar normapur

The individual mycotoxin solid calibration standards (1 mg) of AFB1, AFB2, AFG1, AFG2 and zearalanone (ZAN) (internal standard) were obtained from Sigma Aldrich (Bornem, Belgium). All mycotoxin solid standards were dissolved in methanol (1 mg/mL), and were storable for a minimum of 1 year at -18 °C. The working solutions of AFB1, AFB2, AFG1, AFG2 and ZAN (10 ng/µl) were prepared in methanol, stored at -18 °C, and renewed monthly. Water was obtained from a Milli-Q ® SP Reagent water system from Millipore Corp. (Brussels, Belgium). Disinfectol ® (denaturated ethanol with 5 % ether) was supplied by Chem-Lab (Zedelgem, Belgium).
Methanol (LC-MS grade) was purchased from BioSolve (Valkenswaard, the Netherlands), while acetonitrile (Analar Normapur) and ammonium acetate were obtained from VWR International (Zaventem, Belgium). Acetic acid (glacial, 100 %) was supplied by Merck (Darmstadt, Germany). MultiSep ® 228 AflaPat columns were purchased from Romer Labs (Tulln, Austria).
Protease (Aspergillus oryzae, 500 U/g), α-amylase (Aspergillus oryzae, 30 U/mg) and cellulase (Aspergillus niger, 0.3 U/mg) were purchased from Sigma Aldrich (Bornem, Belgium), as TFMSA (≥ 99 %) and KOH (≥ 99 %). sodium acetate buffer (SAB) was prepared with sodium acetate (27.21 g, ≥ 99 % from Sigma Aldrich), glacial Acetic acid (6 mL) and Milli-Q water (994 mL), and the pH was adjusted to 5.0 with NaOH (10 M).