Stably Cas9-expressing HepG2 cell line (catalog no.: T3256), lentiviral sgRNA targeting human LDLR (catalog no.: 264181110204), and nonviral plasmids containing sgRNA targeting individual candidate genes were purchased from ABM Goods, Inc (Canada). Whole-genome lentiviral pooled CRISPR-Cas9 KO library (catalog no.: 73178-LV) was obtained from Addgene (Cambridge, MA). Alexa Fluor™ 568 carboxylic acid succinyl ester, Alexa Fluor™ 488-Human Transferrin conjugate (catalog no.: T13342), and DiI-human LDL conjugate (catalog no.: L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-quantitative PCR (qPCR) were purchased from Applied Biosystems (Foster City, CA). Monensin, sodium salt (catalog no.: M5273), was purchased from Sigma-Aldrich (St. Louis, MO). Anti-LDLR antibody (catalog no.: 3839) was purchased from BioVision (Milpitas, CA), anti-TAGLN antibody (catalog no.: GT336) from Invitrogen (Carlsbad, CA), and anti-PCSK7 antibody (catalog no.: 19346S) from Cell Signaling Technology (Danvers, MA).
Anti pcsk7 antibody
Manufactured by Cell Signaling Technology
The Anti-PCSK7 antibody is a laboratory reagent designed for the detection and analysis of PCSK7 protein in various samples. PCSK7 is a proprotein convertase enzyme involved in the proteolytic processing of protein precursors. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of PCSK7 in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 human transferrin conjugate, by Thermo Fisher Scientific (2 mentions)
Dii human ldl conjugate, by Thermo Fisher Scientific (2 mentions)
Anti tagln antibody, by Thermo Fisher Scientific (2 mentions)
Anti ldlr antibody, by Abcam (2 mentions)
Monensin sodium salt, by Merck Group (2 mentions)
Alexa fluor tm 568 carboxylic acid succinyl ester, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti pcsk7 antibody
1
High-throughput LDLR Regulation Screening
2
CRISPR/Cas9-Mediated Functional Screening
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Stably Cas9-expressing HepG2 cell line (Cat# T3256), lentiviral sgRNA targeting human LDLR (Cat# 264181110204) and non-viral plasmids containing sgRNA targeting individual candidate genes were purchased from ABM Goods, Inc. (Canada). Wholegenome lentiviral pooled CRISPR/Cas9 knockout library (Cat# 73178-LV) was obtained from Addgene (Cambridge, MA). Alexa Fluor TM 568 carboxylic acid succinyl ester, Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI-human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City, CA). Monensin, sodium salt, (Cat# M5273) was purchased from Sigma-Aldrich (St. Louis, MO). Anti-LDLR antibody (Cat# 3839) was purchased from BioVision (Milpitas, CA), anti-TAGLN antibody (Cat# GT336) from Invitrogen (Carlsbad, CA), and anti-PCSK7 antibody (Cat# 19346S) from Cell Signaling Technology (Danvers, MA).
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