Normal ovarian cells IOSE80 were cultured in RMPI‐1640 medium (Corning) containing 10% foetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd.) and 1% Penicillin–Streptomycin (Anhui Biosharp Biotechnology Co., Ltd.), incubated in an incubator at 37°C with 5% CO2, and passed every 2–3 days. EOC cells SKOV3 were cultured in McCoy's 5A medium (Shanghai BasalMedia Biotechnology Co., Ltd.). EOC cells A2780 were cultured in the medium RPMI‐1640, with the addition of 2% glutamine (Wuhan Procell Life Science & Technology Co., Ltd.), and the other conditions were the same as above. IOSE80 and A2780 was derived from EXPASY. SKOV3 was derived from ATCC. All the cells were purchased from suyan biotech.
Foetal bovine serum (fbs)
Manufactured by Zhejiang Tianhang Biotechnology
Sourced in United States
Foetal bovine serum is a cell culture supplement derived from the blood of bovine fetuses. Its core function is to provide a rich source of growth factors, nutrients, and other essential components required for the optimal growth and proliferation of cells in in vitro cell culture systems.
Automatically generated - may contain errors
2 protocols using foetal bovine serum (fbs)
1
Culturing Ovarian Cell Lines IOSE80, SKOV3, and A2780
2
Culturing Rat Cardiomyocyte Cell Line H9c2
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The rat cardiomyocyte cell line H9c2 was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.
H9c2 cells were cultured in DMEM (Gibco, USA) containing 10% foetal bovine serum (Zhejiang Tianhang Biotechnology, China), 100 U/mL penicillin and 100 g/mL streptomycin. The cells were cultured in a CO2 constant-temperature incubator with a 5% volume fraction at 37 °C.
H9c2 cells were cultured in DMEM (Gibco, USA) containing 10% foetal bovine serum (Zhejiang Tianhang Biotechnology, China), 100 U/mL penicillin and 100 g/mL streptomycin. The cells were cultured in a CO2 constant-temperature incubator with a 5% volume fraction at 37 °C.
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