Nanospect ctplus camera

Mice were subcutaneously inoculated into the right flank with 9Â10 6 of MC38-FAP cells. When tumors reached the size of about 500 mm 3 , mice were injected with approximately 6 MBq of indium-111-conjugated DARPin molecules, corresponding to 100 kBq per mg of DARPin molecules, into the tail vein.
Single-photon emission computed tomography (SPECT)/X-ray computed tomography (CT) images were performed with the NanoSPECT/CTPlus camera (version 1.2, Bioscan). Acquisitions of SPECT and CT were performed with the software Nucline (version 1.02). The CT was also reconstructed with the software Nucline, whereas for SPECT the software HISPECT was used (version 1.4.3049, Scivis GmbH). Fusions of SPECT and CT data were analyzed with the VivoQuant postprocessing software (Version 3.5, Invicro). Whole-body activity was measured in a gamma counter tube prior to imaging acquisition. SPECT/CT in vivo images were taken from anesthetized mice at time points 4, 24, 48, 72 and 96 hours after injection of 111 In-DARPin constructs. The 3D segmentation tool of the VivoQuant postprocessing software was used for quantification of the measured radioactivity in the tumors.

According to the experimental paradigm (Fig. 1), 99m Tc-DTPA (20 MBq/100 mL), 99m Tc-annexin-V-128 (37 MBq/150 mL), and 99m Tc-HMPAO (20 MBq/150 mL) was injected through the tail vein on days 2, 3, and 14, respectively, after MCAO. The animals were Twelve hours after injection of the radiolabeled cells, ECFC homing was assessed using SPECT/CT. Two, 3, and 14 d after MCAO, BBB disruption was assessed using 99m Tc-DTPA, apoptosis using 99m Tc-annexin-V-128, and CBF using 99m Tc-HMPAO, respectively. Fourteen days after MCAO, neuronal survival and astrocyte proliferation were evaluated ex vivo using immunohistochemistry.
imaged under a NanoSPECT/CT PLUS camera (Bioscan) 30 min after 99m Tc-DTPA or 99m Tc-HMPAO injection and 90 min after 99m Tc-annexin-V-128 injection. Multipinhole SPECT parameters were set up as follows: termination condition, 10,000 counts; picture size, 256 • 256; zoom factor, 1.14; and pixel size, 1.00 mm 2 .