ALT-803 was generously provided by Altor BioScience Corporation and was diluted in sterile PBS for in vitro and in vivo studies. Recombinant human IL-15 (Novoprotein), human IL-2, human IL-21, mouse IL-7 (Peprotech), human IL-27 (eBioscience), and Concanavalin A (Type VI, Sigma-Aldrich) were reconstituted in sterile PBS and stored at −20°C. Trametinib (GSK-1120212) was purchased from LC Laboratories and suspended in vehicle solution of 10% PEG-300 (Sigma Aldrich) and 10% Cremophor EL (EMD Millipore) in sterile dH20 for in vivo oral gavage experiments. For in vitro assays, all inhibitors were dissolved in sterile DMSO and diluted in the assays 1:1000, so that the final concentration of DMSO was 0.1%.
Trametinib gsk 1120212
Manufactured by LC Laboratories
Sourced in United States
Trametinib (GSK-1120212) is a small-molecule inhibitor that targets the mitogen-activated protein kinase (MAPK) pathway by inhibiting MEK1 and MEK2. It is a white to off-white powder that is soluble in DMSO and dimethyl formamide.
Automatically generated - may contain errors
Lab products found in correlation
Human il 2, by Thermo Fisher Scientific (1 mentions)
Peg300, by Merck Group (1 mentions)
Human il 21, by Thermo Fisher Scientific (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Mouse il 7, by Thermo Fisher Scientific (1 mentions)
Cremophor el, by Merck Group (1 mentions)
A 1210477, by Merck Group (1 mentions)
Abt 263, by Selleck Chemicals (1 mentions)
Mk 2206, by Selleck Chemicals (1 mentions)
Everolimus, by Selleck Chemicals (1 mentions)
At7867, by Selleck Chemicals (1 mentions)
Dasatinib, by LC Laboratories (1 mentions)
3 protocols using trametinib gsk 1120212
1
Reconstitution and Preparation of Immunotherapeutic Agents
2
Exploring Anticancer Drug Compounds
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Dasatinib (BMS-354825) and trametinib (GSK-1120212) were purchased from LC Laboratories (Woburn, MA, USA); MK2206 and ABT-263 were purchased from Selleckchem (Houston, TX, USA). A-1210477 was purchased from Sigma Aldrich (St. Louis, MO, USA). All drugs were dissolved in dimethyl sulfoxide at 10 mM stocks.
3
Compound Screening Assay Protocol
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For the drug screening assays, AT7867 and Everolimus were purchased from SelleckChem, trametinib (GSK-1120212) and dasatinib (BMS-354825) were purchased from LC laboratories. The drugs were dissolved in dimethyl sulfoxide.
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