Keratin 14 prb 155p

Manufactured by Fortrea

Sourced in United States, Japan

Keratin 14 (PRB-155P) is a lab equipment product used for research purposes. It is a purified recombinant protein that can be used in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Podoplanin, by Thermo Fisher Scientific (1 mentions) F actin, by Thermo Fisher Scientific (1 mentions) α tubulin dm1a, by Merck Group (1 mentions) G actin, by Merck Group (1 mentions) Destrin, by Abcam (1 mentions) Alexa fluor 488 546 647, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Prb 440p, by Fortrea (1 mentions)

2 protocols using keratin 14 prb 155p

Immunohistochemical Characterization of Murine Tissues

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Tissue samples from mice were fixed in 10% (w/v) formaldehyde, embedded in paraffin, and stained with HE for histological examination. For immunohistochemistry, tissue samples were fixed via HOPE Fixative (Polysciences, Inc., Warrington, PA, USA). The specimens were embedded in paraffin. Immunohistochemistry was performed using avidin-biotin-peroxidase complex (ABC). Monoclonal anti-AID antibody (MAID-2) was described [16 (link)]. Other antibodies were purchased: keratin 5 (sc-17090; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), ΔN p63 (sc-8609; Santa Cruz Biotechnology), Clara cell 10kd protein (CC-10, sc-9772; Santa Cruz Biotechnology), epithelial cadherin (E-cadherin, 24E10; Cell Signaling, Danvers, MA, USA), leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5, ab75732; Abcam, Cambridge, MA, USA), surfactant protein C (SP-C, sc-13976; Santa Cruz Biotechnology), Lgr6 (sc-99123; Santa Cruz Biotechnology), keratin 14 (PRB-155P; Covance, Berkeley, CA, USA), and podoplanin (53–5381; eBioscience, San Diego, CA, USA). Each section was counterstained with hematoxylin.

Kitamura J., Uemura M., Kurozumi M., Sonobe M., Manabe T., Hiai H., Date H, & Kinoshita K. (2015). Chronic Lung Injury by Constitutive Expression of Activation-Induced Cytidine Deaminase Leads to Focal Mucous Cell Metaplasia and Cancer. PLoS ONE, 10(2), e0117986.

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Multi-Antibody Immunofluorescence Imaging

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Primary antibodies used were: Wdr1 (G-13, Santa Cruz), 1:100; Destrin (ab11072, abcam), 1:100; cofilin (ab42824, abcam), 1:5,000; HPRT (ab10479, abcam), 1:5,000; GAPDH (ab8245, abcam), 1:5,000; GFP (ab13970), 1:2,000; myosin II (PRB-440P, Covance), 1:200; pericentrin (PRB-432C, Covance), 1:1,000; E-cadherin (ECCD-1, M. Takeichi, RIKEN, Japan), 1:500; CD104 (β4 integrin, 553745 BD-Pharmingen), 1:500; P-cadherin (P-cad, Zymed), 1:1,000; Keratin14 (PRB-155P, Covance), 1:1,000; keratin 10 (PRB-159P, Covance), 1:1,000; Celsr1 (Fuchs lab), 1:250; keratin 6 (Rb 415, Millipore), 1:500; PAR3 (07-330, Millipore), 1:100; phospho-S3-Cofilin (77G2, Cell Signaling), 1:5,000; Rab11 (D4F5, Cell Signaling), 1:500; β-actin (AC-15, Sigma), 1:5,000; γ-actin (2-2.1.14.17, Sigma), 1:5,000; α-tubulin (DM1A, Sigma), 1:1,000; acetylated tubulin (6-11B-1, Sigma), 1:1,000. Secondary antibodies conjugated to FITC, rhodamine (Jackson Immunoresearch), or Alexa Fluor 488/546/647 (Invitrogen) were diluted 1:1,000 (sagittal sections) or 1:200 (whole mount), and samples were incubated 1 h–overnight. F-actin was labelled with phalloidin–Alexa Fluor 546/647 (Invitrogen).

Luxenburg C., Heller E., Pasolli H.A., Chai S., Nikolova M., Stokes N, & Fuchs E. (2015). Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity. Nature cell biology, 17(5), 592-604.

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