Ethyl acetate for high performance liquid chromatography

Tofacitinib citrate, voriconazole, hydrocortisone (an internal standard for tofacitinib analysis), and polyethylene glycol (PEG) 400 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Heparin and 0.9% NaCl injectable solutions were purchased from JW Pharmaceutical Corporation (Seoul, Korea). Ethyl acetate for high-performance liquid chromatography (HPLC) analysis and β-cyclodextrin were purchased from J.T. Baker (Phillipsburg, NJ, USA) and Wako (Osaka, Japan), respectively. The β-nicotinamide adenine dinucleotide phosphate hydrogen (NADPH)-generating system was obtained from Corning Life Sciences (Woburn, MA, USA). Primary CYP2C11 and CYP3A1/2 antibodies were kindly donated by Detroit R&D Inc. (Detroit, MI, USA) and β-actin was purchased from Cell Signaling Technology (Beverly, MA, USA). Secondary goat, rabbit, and mouse antibodies were purchased from Bio-Rad (Hercules, CA, USA). All other chemicals and reagents were of analytical or HPLC grade and used without further purification.

Tofacitinib citrate and hydrocortisone (an internal standard) were obtained from Sigma Aldrich (St. Louis, MO, USA), and ethyl acetate for high-performance liquid chromatography (HPLC) analysis was purchased from J.T. Baker (Phillipsburg, NJ, USA). Gentamicin and cisplatin were obtained from Shin Poong Pharmaceutical (Seoul, Korea) and Tokyo Chemical Industry (Tokyo, Japan), respectively. Heparin and 0.9% NaCl-injectable solution were purchased from JW Pharmaceutical Corporation (Seoul, Korea), and β-cyclodextrin is a product of Wako (Osaka, Japan). Primary antibodies to CYP2B1/2, CYP1A1/2, CYP2D1, CYP2C11, CYP2E1, and CYP3A1/2 were produced by Detroit R&D Inc. (Detroit, MI, USA). β-actin was purchased from Cell Signaling Technology (Beverly, MA, USA). Secondary goat, rabbit, and mouse antibodies were purchased from Bio-Rad (Hercules, CA, USA). All other chemicals and reagents were analysis- or HPLC-grade and used without further purification.