Total RNA from PBMC samples was extracted using Tripure Reagent (Roche Diagnostics, Barcelona, Spain) and then purified with E.Z.N.A. MicroElute RNA Clean Up (Omega Biotek, Vermont, USA), followed by precipitation with 3M sodium acetate and absolute ethanol. Moreover, total RNA from retroperitoneal adipose and liver samples was extracted using Tripure Reagent (Roche Diagnostics, Barcelona, Spain) and purified by precipitation with 3M sodium acetate and absolute ethanol. RNA yield was quantified using a NanoDrop ND 1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). The integrity of PBMC samples was measured on an Agilent 2100 Bioanalyzer with RNA 6000 Nano chips (Agilent Technologies, South Queensferry, United Kingdom). The integrity of retroperitoneal adipose tissue and liver RNA samples was confirmed using agarose gel electrophoresis.
Microelute rna clean up
Manufactured by Omega Bio-Tek
Sourced in United States
The MicroElute RNA Clean Up is a lab equipment product designed for the purification of RNA samples. It utilizes a micro-spin column format to efficiently remove contaminants and impurities from RNA samples, allowing for high-quality RNA recovery.
Automatically generated - may contain errors
4 protocols using microelute rna clean up
1
Extraction and Purification of Total RNA
2
Total RNA Extraction from Adipose Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA from tPVAT and IAT samples was extracted using Tripure Reagent (Roche Diagnostics, Barcelona, Spain). RNA samples were purified with E.Z.N.A. MicroElute RNA Clean Up (Omega Bio-tek, VT, United States), and with 3M sodium acetate and absolute ethanol. RNA yield was quantified using a NanoDrop ND 1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, United States) and the integrity was measured on an Agilent 2100 Bioanalyzer with RNA 6000 Nano chips (Agilent Technologies, South Queensferry, United Kingdom).
3
RNA Extraction from PBMC Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA from PBMC samples was extracted using Tripure Reagent (Roche Diagnostics) and then purified with E.Z.N.A. MicroElute RNA Clean Up (Omega Bio-tek). RNA yield was quantified in a NanoDrop ND 1000 spectrophotometer (NanoDrop Technologies), and its integrity was confirmed using agarose gel electrophoresis.
4
Isolation and Purification of Total RNA from Rat and Ferret Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from rat and ferret hypothalamus and PBMC using Tripure Reagent (Roche Diagnostics Barcelona, Spain). Total RNA from ferret and rat hypothalamus was purified by precipitation with 3 M sodium acetate and absolute ethanol, while ferret and rat PBMC samples were purified with E.Z.N.A. MicroElute RNA Clean Up (Omega Bio-Tek, Vermont, USA); additionally, ferret PBMC samples were also precipitated with sodium acetate. NanoDrop ND 1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) was used to quantify RNA yield and its integrity was confirmed using agarose gel electrophoresis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!