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2 protocols using anhydrous ethyl alcohol

1

Synthesis and Characterization of Multifunctional Nanoparticles

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The aqueous suspension of carboxylated polystyrene microspheres of average size 0.2 μm and 0.05 μm and poly(diallyldimethylammonium chloride) (PDDA) were purchased from PolySciences, Inc, ammonium hydroxide (NH4OH), potassium carbonate (K2CO3), isopropyl alcohol (IPA), and ammonium carbonate were procured from Fisher Scientific, NJ, USA. Tetraethyl orthosilicate (reagent grade, 98% (TEOS)), chloroauric acid (HAuCl4), tetrakis(hydroxymethyl)phosphonium chloride (80% in water (THPC)), sodium hydroxide (NaOH), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), doxorubicin hydrochloride (DOX), dimethyl sulfoxide (DMSO), P(NIPAM-co-MAA) formaldehyde solution (16%) were purchased from Sigma-Aldrich (St. Louis, MO, USA); Dimethylformamide (DMF) was purchased from Macron Chemicals, PA, USA; iron oxide nanoparticles with carboxylic acid group were purchased from Ocean NanoTech, SD, USA. Anhydrous ethyl alcohol (EtOH) was purchased from JT Baker, NJ, USA. Borosilicate Capillary tube (0.86 mm OD) was obtained from Warner Instruments, CT, USA and polydimethylsiloxane (PDMS) Sylgard 184 silicone elastomer kit from Dow Corning, Auburn, USA.
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2

Synthesis of Gold-Coated Polystyrene Nanoparticles

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Carboxylate modified polystyrene (PS-COOH) spheres (100 nm 2.7% in water) were purchased from PolySciences. Tetrakis(hydroxymethyl) phosphonium chloride(THPC, 80% in water) sodium hydroxide(NaOH,10M), and chloroauric acid trihydrate (HAuCl4·3H2O 99.9%) were purchased from Aldrich. Potassium carbonate (K2CO3), formaldehyde (37%) and ammonium hydroxide (NH3OH, 29.79%) were purchased from Fisher Scientific. Anhydrous Ethyl alcohol (EtOH) was purchased from JT Baker. (3-Aminoethylamino)propyl-trimethoxysilane (AEAPTMS), anhydrous isopropanol, tetrahydrofuran (THF), O-(2-Mercaptoethyl)-O′-methylpolyethylene glycol (10 kDa, mPEG-SH) was purchased from Sigma. Deionized (DI) water used in samples was produced using a Millipore Advantage A10 system with a resistance of 18.2 MΩ. Phosphate buffered saline (PBS, 1x) was purchased from Corning.
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