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Annexin 5 fitc detection kit

Manufactured by Keygen Biotech
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Sourced in China

The Annexin V-FITC detection kit is a laboratory reagent used to identify and quantify apoptotic cells. It utilizes the fluorescent protein Annexin V, which binds to phosphatidylserine exposed on the surface of cells undergoing programmed cell death.

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Lab products found in correlation

Facs caliber, by BD (1 mentions) Facscan system, by BD (1 mentions) Facscan flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V-FITC/PI apoptosis detection kit Annexin V-FITC/PI double-stain apoptosis detection kit Annexin V-FITC and PI apoptosis detection kit Annexin V-FITC/Propidium Iodide (PI) double-staining apoptosis detection kit Annexin V-conjugated FITC apoptosis detection kit Annexin V-FITC Staining Cell Apoptosis Detection kit Annexin V-FITC apoptosis detection kit (KGA108), Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit Annexin V-FITC/PI double-staining cell apoptosis detection kit Annexin V-FITC/PI cell apoptosis detection kit

5 protocols using annexin 5 fitc detection kit

1

Apoptosis Evaluation by FACS Analysis

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Apoptosis was assessed by fluorescence-activated cell sorting (FACS) analysis according to the instructions of the Annexin V-FITC detection kit (KeyGEN BioTECH, Nanjing, China). HCT-116 and HT-29 cells were treated with 5 mM NaB or 10 mM NaB for 24 h. Then, all the cells were collected and incubated with FITC-conjugated Annexin V and propidium iodide (PI) dye. Apoptosis was measured by flow cytometry (BD Biosciences Franklin Lakes, NJ, USA). Results were analyzed with CellQuest software.
Yuan Y., Li B., Kuang Y., Ni S., Zhuge A., Yang J., Lv L., Gu S., Yan R., Li Y., Wang K., Yang L., Zhu X., Wu J., Bian X, & Li L. (2020). The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell International, 20, 212.
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2

Annexin V-FITC Apoptosis Assay

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Apoptosis was assessed by uorescence-activated cell sorting (FACS) analysis according to the instructions of the Annexin V-FITC detection kit (KeyGEN BioTECH, Nanjing, China). HCT-116 and HT-29 cells were treated with 5 mM NaB or 10 mM NaB for 24 hours. Then, all cells were collected and incubated with FITC-conjugated Annexin V and propidium iodide (PI) dye. Apoptosis was measured by ow cytometry (BD Biosciences Franklin Lakes, NJ, USA). Results were analyzed with CellQuest software.
Yuan Y., Li B., Liao Y., Ni S., Zhuge A., Yang J., Lv L., Gu S., Ren Y., Li Y., Wang K., Yang L., Zhu X., Wu J., Bian X., & Lan-juan L. (2020). Fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells.
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3

Apoptosis Analysis of Liver Cancer Cells

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Apoptosis was determined by fluorescence activated cell sorting (FACS) analysis using the Annexin V‐FITC detection kit (KeyGEN BioTECH, Nanjing, China) as described by the manufacturer. HUH‐7, 7721 and HEPG2 cell lines were plated in six‐well tissue plates (4 × 105 cells/well) and incubated overnight. Proliferating cells were treated with or without bazedoxifene for 12 hours. Cells were trypsinized and centrifuged at 720 g for 5 minutes. After washing twice with PBS, the cells were then harvested and incubated with fluorescein isothiocyanate‐conjugated Annexin V and propidium iodide dye (PI) following the manufacturer's protocol before evaluation by flow cytometry (FACS Caliber; BD Biosciences Franklin Lakes, NJ, USA). CellQuest software was used to analyze apoptosis.
Ma H., Yan D., Wang Y., Shi W., Liu T., Zhao C., Huo S., Duan J., Tao J., Zhai M., Luo P., Guo J., Tian L., Mageta L., Jou D., Zhang C., Li C., Lin J., Lv J., Li S, & Lin L. (2019). Bazedoxifene exhibits growth suppressive activity by targeting interleukin‐6/glycoprotein 130/signal transducer and activator of transcription 3 signaling in hepatocellular carcinoma. Cancer Science, 110(3), 950-961.
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4

Annexin V-FITC Cell Apoptosis Assay

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Cells were stained with an Annexin V-FITC detection kit (KeyGen, Nanjing, Jiangsu, China), following the manufacturer’s instructions, and analyzed with a BD FACScan system (BD Biosciences, San Jose, CA, USA). The graph was plotted using Flowjo 7.6.5 software (FLOWJO LLC, Ashland, KY, USA).
Yao Y.H., Cui Y., Qiu X.N., Zhang L.Z., Zhang W., Li H, & Yu J.M. (2016). Attenuated LKB1-SIK1 signaling promotes epithelial-mesenchymal transition and radioresistance of non–small cell lung cancer cells. Chinese Journal of Cancer, 35, 50.
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5

Apoptosis Detection via Flow Cytometry

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Flow cytometry was performed to detect the cell apoptosis. Cells under certain treatments for 24 h were collected and washed with PBS buffer. Then cells were stained with fluorescein isothiocyanate (FITC)-conjugated annexin V and propidium iodide (PI) using Annexin V-FITC detection kit (KeyGen, Nanjing, Jiangsu, China) according to the manufacturer's instructions. Then the apoptotic cells were analyzed with BD FACScan flow cytometer (BD Biosciences, San Diego, CA, USA). The experiments were performed in triplicate.
Kou B., Wang X.D., Sun X.P., Qi Q., Yang M., Yun Y.N., Zhou J.S, & Liu W. (2022). LKB1 inhibits proliferation, metastasis and angiogenesis of thyroid cancer by upregulating SIK1. Journal of Cancer, 13(9), 2872-2883.
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