Cell lines were cultured in RPMI-1640 medium (Gibco®) supplemented with 10% fetal bovine serum (FBS), 50 µg/mL penicillin/streptomycin and 2 mM L-glutamine at 37 °C in a humidified atmosphere (5% CO2). Ramos cell line derives from a Burkitt’s lymphoma and it was obtained from American Type Culture Collection (ATCC). Ramos cells were stably transfected with a vector encoding for ZAP-70 protein fused with Green fluorescent protein (GFP) or GFP only as a control24 (link). Jurkat cells are derived from a T-cell acute lymphoblastic leukemia and they were obtained from ATCC. Jurkat cells were stimulated with pervanadate (3 mM H2O2/1 mM NaVO4) for 5 minutes at 37 °C, as a positive control for phosphoproteins. Human Bone Marrow Stromal Cell (BMSC) cell line UE6E7T-2 was provided by Riken Cell Bank (Ibaraki, Japan) and cultured at 37 °C in 5% CO2 atmosphere in Dulbecco’s Modified Eagle Medium (DMEM; Gibco®) supplemented with 2 mM L-glutamine, 10% heat-inactivated FBS and 50 µg/mL penicillin/streptomycin.
Ramos cell line
Manufactured by American Type Culture Collection
Sourced in United States
The Ramos cell line is a human B lymphoblast cell line derived from a Burkitt's lymphoma patient. It is commonly used in cell biology research as a model for B-cell malignancies.
Automatically generated - may contain errors
Lab products found in correlation
Ue6e7t 2, by RIKEN Cell Bank (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Rpmi 1640, by Corning (1 mentions)
3 protocols using ramos cell line
1
Cell line culture and transfection protocol
2
Cell Culture Protocols for Ramos and BaF3 Cell Lines
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Ramos cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). Ramos cells and BaF3 isogenic cell lines cultured in RPMI 1640 (Corning), containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA), 1% penicillin-streptomycin (Gibco, BRL). 293T cells was cultured in DMEM (Corning), containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA), 1% penicillin-streptomycin (Gibco, BRL).
3
Cell Line Culturing Protocols
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Human pancreatic ductal adenocarcinoma cell line PL45, hTERT-HPNE cell line, CCRF-CEM cell line and Ramos cell line were obtained from the American Type Culture Collection (ATCC). Gastric cancer cell line (MGC-803), breast cancer cell lines (MCF-7 and MDA-MB-231), lung cancer cell lines (H1299, 95-C and 95-D), colon carcinoma cell line (HCT116), melanoma cell line (SK23), human embryonic kidney epithelial cell line (HEK293) and human embryonic lung fibroblast cell line (MRC-5) were obtained from the cell bank of Xiangya Hospital (Changsha, China). All cell lines were cultured at 37 °C in a humid atmosphere with 5% CO2. PL45 cells, MDA-MB-231 cells, MCF-7 cells, MRC-5 cells, SK23 cells, HEK293 cells, DU145 cells and 95-C cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS, Hyclone) and 100 U/mL penicillin-streptomycin. MGC-803 cells, HCT116 cells, H1299 cells, 95-D cells, CCRF-CEM cells, Ramos cells and HL60 cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 100 U/mL penicillin-streptomycin. 75% glucose-free DMEM medium (with additional 2 mM L-glutamine and 1.5 g/L sodium bicarbonate) and 25% M3:Base™ were mixed and then supplemented with 5% FBS, 10 ng/mL human recombinant EGF, 5.5 mM D-glucose and 750 ng/mL puromycin to make the complete growth medium for culturing hTERT-HPNE cells.
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