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Atcc tib 202

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ATCC TIB-202 is a cell line derived from murine B lymphocytes. It is a widely used research tool for the study of B cell biology and immune system function.

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Lab products found in correlation

Phorbol myristate acetate (pma), by Merck Group (5 mentions) Rpmi 1640 medium, by Merck Group (3 mentions) Thp 1, by American Type Culture Collection (3 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Ez pcr mycoplasma test kit, by Sartorius (2 mentions) Penicillin streptomycin antibiotic, by Thermo Fisher Scientific (2 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Euroclone (1 mentions) Atcc crl 1740, by American Type Culture Collection (1 mentions) Atcc htb 37, by American Type Culture Collection (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Thp 1, by Thermo Fisher Scientific (1 mentions) Pcs 300 030, by American Type Culture Collection (1 mentions) Raw264.7 cells atcc tib 71, by American Type Culture Collection (1 mentions) Human monocyte isolation kit 2, by Miltenyi Biotec (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Thp1 xblue cells, by InvivoGen (1 mentions)

Close spelling variants of this product

TIB-202 (277 citations) TIB-202TM (23 citations) THP-1 ATCC® TIB-202™) (18 citations) ATCC® TIB-202TM (4 citations) THP-1 cells (ATCC® TIB-202) (4 citations) Human THP-1 (ATCC TIB-202) (2 citations)

36 protocols using atcc tib 202

1

Investigating Inflammatory Responses in Caco-2 and THP-1 Cells

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Caco-2 human epithelial colorectal adenocarcinoma cells (ATCC® HTB-37) and human THP-1 monocytes (ATCC® TIB-202™) were purchased from ATCC (Manassas, VA, USA). High glucose Dulbecco’s Modified Eagle Medium (DMEM), Hanks’ Balanced Salt Saline (HBSS), non-essential amino acids (NEAA), L-glutamine, penicillin–streptomycin mix and CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS) were purchased from Promega (Madison, WI, USA). Caco-2 human colon adenocarcinoma cell line (ATCC® HTB-37™), LNCaP androgen-sensitive human prostate adenocarcinoma cell line (ATCC® CRL-1740™) and THP-1 (ATCC® TIB-202™) were purchased from ATCC (Manassas, VA, USA). Roswell Park Memorial Institute (RPMI) 1640 Medium, lipopolysaccharide (LPS), diclofenac, were purchased from Sigma-Aldrich (St Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Euroclone (Milan, Italy). Interleukin 1β (IL-1β) ELISA kit was purchased from R and D Systems (Minneapolis, MN, USA). Proinflammatory cytokine-specific array was purchased from Ray Biotech (Peachtree Corners, GA, USA).
Murzilli S., Mirone V., Micheletto M., Tedesco E., Maira G.D., Benetti F, & Vanelli A. (2023). Evaluation of the Immunomodulatory Effects of a Probiotics and Natural Extract-Based Formulation in Bacterial-Induced Prostatitis. Life, 13(2), 389.
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2

Macrophage Differentiation and Nanoparticle Exposure

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Human monocytic THP-1 cells (ATCC TIB-202) were purchased from ATCC (LGC Standards, Molsheim, France) and cultured in DMEM medium (supplemented with 10% fetal calf serum (FCS) and 100 U/ml Penicillin/Streptomycin). Prior to exposure, the cells were seeded a concentration of 2.5 × 105 cells/cm2 in DMEM medium with 30 ng/ml of PMA (Sigma-Aldrich, France) to induce macrophage differentiation for 24 hr28 (link),29 (link). The cells were then washed 3x with serum-free DMEM medium and exposed to NP (25 µg/cm2, non-cytotoxic, data not shown14 (link)) for 24 hr. Following exposure, the cells were washed before being harvested for RNA isolation.
Audureau É., Simon-Deckers A., Franco-Montoya M.L., Annangi B., Kermanizadeh A., Boczkowski J, & Lanone S. (2018). Substantial modification of the gene expression profile following exposure of macrophages to welding-related nanoparticles. Scientific Reports, 8, 8554.
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3

Cigarette Smoke Extract Impacts on Airway Cells

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The human monocytic cell line THP-1 (ATCC® TIB-202™) was purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). THP-1 cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin. Davidoff cigarettes containing 10 mg tar and 0.8 mg nicotine per cigarette were used for preparing the cigarette smoke extract (CSE) according to previous methods [20 (link), 21 (link)] with modifications. THP-1 cells were treated with normal medium, 2.5% CSE, 25 μg ovalbumin (OVA), or CSE (2.5%) plus OVA (25 μg) for 48 h.
Primary normal human bronchial epithelial (NHBE) cells (ATCC® PCS-300-010™) obtained from ATCC were cultured in ATCC Airway Epithelial Cell Basal Medium (ATCC® PCS-300-030™) supplemented with Bronchial Epithelial Cell Growth Kit (ATCC® PCS-300-040™). NHBE cells were treated with CSE or OVA or both in the same manner as THP-1 cells.
Gene expression was measured using qRT-PCR with a Taqman probe (Thermo Fisher Scientific) and specific primers. The relative expression was calculated using the 2−∆∆Ct method.
Chang Y.P., Tsai Y.H., Chen Y.M., Huang K.T., Lee C.P., Hsu P.Y., Chen H.C., Lin M.C, & Chen Y.C. (2024). Upregulated microRNA-125b-5p in patients with asthma-COPD overlap mediates oxidative stress and late apoptosis via targeting IL6R/TRIAP1 signaling. Respiratory Research, 25, 64.
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4

Culturing Anaerobic Oral Bacteria and Immune Cells

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Porphyromonas gingivalis 33277 was cultured anaerobically in trypticase soy broth supplemented with yeast extract (1 mg/ml), hemin (5 μg/ml) and menadione (1 μg/ml). S. sanguinis SK36 (from Dr. Kitten’s lab) and S. gordonii DL1 were grown at 37°C in Anoxomat jars (Spiral Biotech) under microaerobic conditions (7% H2, 7% CO2, 80% N2, and 6% O2) in brain-heart infusion (BHI; Bacto, Sparks, MD) broth. PBMCs were obtained from healthy donors according to protocols approved by the University of Louisville Institutional Review Board. Monocytes were isolated by negative selection using a Human Monocyte Isolation Kit II from Miltenyi Biotec (130-091-153). For bacterial treatment, monocytes isolated from PBMCs were directly seeded in either 96- or 6-well plates. RAW 264.7 (ATCC® TIB-71™) cells were purchased from ATCC and maintained in Dulbecco’s modified Eagle’s medium (Invitrogen) containing 10% fetal bovine serum and penicillin-streptomycin (100 U/mL). Human monocytic leukemia cells (THP-1) were purchased from ATCC (ATCC® TIB-202™) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (HyClone) and penicillin-streptomycin (100 U/mL). Cells were cultured at a temperature of 37°C in a humidified growth chamber under 5% CO2.
Lü L., Yakoumatos L., Ren J., Duan X., Zhou H., Gu Z., Mohammed M., Uriarte S.M., Liang S., Scott D.A., Lamont R.J, & Wang H. (2020). JAK3 restrains inflammatory responses and protects against periodontal disease through Wnt3a signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(7), 9120-9140.
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5

Differentiation of THP-1 Monocytes into Macrophages

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Human peripheral blood monocyte cell line THP-1 (ATCC© TIB-202™) ((ATCC, Manassas, VA, USA) cells were cultured in an RPMI-1640 medium (Gibico, Shanghai, China) supplemented with 10% fetal bovine serum (FBS) (Gibico, Carlsbad, CA, USA) and maintained at 37 °C in a humidified 5% CO2 atmosphere. We exploited the method of THP-1 macrophage differentiation [40 (link),41 (link)] and the THP-1 monocytes were treated with 7.5 nM (5 ng/mL) phorbol myristate acetate (PMA) (Sigma, Saint Louis, MO, USA) for 48 h. Cells were cultured in a fresh completed medium without PMA for 24 h before being infected with M. pneumoniae.
Ma C., Hao X., Gao L., Wang Y., Shi J., Luo H, & Li M. (2023). Extracellular Vesicles Released from Macrophages Infected with Mycoplasma pneumoniae Stimulate Proinflammatory Response via the TLR2-NF-κB/JNK Signaling Pathway. International Journal of Molecular Sciences, 24(10), 8588.
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6

Culturing THP-1 and THP1-XBlue Cells

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THP-1 cells (ATCC® TIB-202™) have been purchased from ATCC® (Manassas, VA 20110 United States), while THP1-XBlue™ cells have been purchased from InvivoGen (InvivoGen, San Diego, CA, United States). Both cell lines were cultured in RPMI 1640, 2 mM L-glutamine, 10% heat-inactivated fetal bovine serum, 100 U/ml-100 μg/ml Pen-Strep. Cells were maintained in a humidified 37°C, 5% CO2 incubator. Cells were subcultured every 2 days. Exponentially growing cells were adjusted to 0.5 × 106/ml according to the routine procedure. To maintain selection pressure, 100 μg/ml of Zeocin™ (InvivoGen, San Diego, CA, United States) were added to the growth medium of THP1-XBlue™ cells every other passage. Media and supplements were purchased from Euroclone (Euroclone S.p.A., Pero, Milan, Italy) unless otherwise stated.
Artusa V., Ciaramelli C., D’Aloia A., Facchini F.A., Gotri N., Bruno A., Costa B., Palmioli A., Airoldi C, & Peri F. (2022). Green and Roasted Coffee Extracts Inhibit Interferon-β Release in LPS-Stimulated Human Macrophages. Frontiers in Pharmacology, 13, 806010.
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7

Cytotoxicity Evaluation of C. alba Extract

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The cytotoxicity test was carried out with TPH-1 cells (ATCC® TIB-202™) from the Federal University of Latin American Integration (UNILA) stock (Paraná, Brazil) exposed to the root extract of C. alba, and rutin was found to be one of the major constituents. The extract and rutin were dissolved in DMSO and diluted in RPMI culture medium (Sigma–Aldrich®, São Paulo, SP, Brazil) to prepare a stock solution [44 (link)]. Once attached, the culture medium was removed and sample solutions were added at concentrations of 0.87, 1.30, 1.70, and 2.12 μg/mL. The final volume in each well was 100 µL, and the number of cells present in each well was 1 × 104 cells. The plates were incubated for 48 h at 37 °C in a humidified atmosphere containing 5% CO2. Next, 100 μL of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and incubated for 4 h. The absorbance at 540 nm was measured using a microplate spectrophotometer (Quimis®, Diadema, SP, Brazil). The assays were performed in triplicate.
Borges J.C., Haddi K., Valbon W.R., Costa L.T., Ascêncio S.D., Santos G.R., Soares I.M., Barbosa R.S., Viana K.F., Silva E.A., Moura W.S., Andrade B.S., Oliveira E.E, & Aguiar R.W. (2022). Methanolic Extracts of Chiococca alba in Aedes aegypti Biorational Management: Larvicidal and Repellent Potential, and Selectivity against Non-Target Organisms. Plants, 11(23), 3298.
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8

Cell Culture of Vero E6, THP-1, and CD16.NK-92

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Vero E6 cells were purchased from ATCC (ATCC VERO C1008), grown at 37C, 5% CO2 and maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, 1% non-essential amino acids. THP-1 cells were purchased from ATCC (ATCC TIB-202), grown at 37C, 5% CO2 and maintained in RPMI-1640 supplemented with 10% fetal bovine serum, 2mM L-glutamine, 10mM HEPES, and 0.05 mM β-mercaptoethanol. CD16.NK-92 were purchased from ATCC (ATCC PTA-6967), grown at 37C, 5% CO2 and maintained in in MEM-α supplemented with 12.5% FBS, 12.5% horse serum, 1.5g/L sodium bicarbonate, 0.02mM folic acid, 0.2mM inositol, 0.1 mM 2-β-mercaptoethanol, 100U/mL recombinant IL-2.
Bates T.A., Lu P., Kang Y.J., Schoen D., Thornton M., McBride S.K., Park C., Kim D., Messer W.B., Curlin M.E., Tafesse F.G, & Lu L.L. (2022). BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with age. Cell Reports, 41(4), 111544.
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9

Cell Culture Protocols for CHO-K1 and THP-1 Cells

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CHO-K1 Chinese hamster ovary cells (ATCC CCL-61) and THP-1 human monocytic cells (ATCC TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and tested for the absence of mycoplasma contamination by Hoechst stain. CHO cells were grown in F12K medium (GIBCO Invitrogen, Grand Island, NY) supplemented with 10% fetal calf serum (FCS) (GIBCO Invitrogen, Grand Island, NY) and antibiotic antimycotic solution (ATB, 0.1 mg/ml streptomycin, 1000 U/ml penicillin and 0.25 µg/ml amphotericin; Sigma-Aldrich, St. Louis, MO). THP-1 cells were cultured in RPMI 1640 (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal calf serum and antibiotic antimycotic solution.
Osicka R., Osickova A., Hasan S., Bumba L., Cerny J, & Sebo P. (2015). Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. eLife, 4, e10766.
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10

THP-1 Cell Culture Protocol

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THP-1 cells were purchased from ATCC (ATCC® TIB-202), were grown at 37°C, 5% CO2 and were maintained in RPMI with 10% fetal bovine serum, penicillin/streptomycin, L-glutamine, HEPES, and beta-mercaptoethanol.
Pullen K.M., Atyeo C., Collier A.R., Gray K.J., Belfort M.B., Lauffenburger D.A., Edlow A.G, & Alter G. (2021). Selective functional antibody transfer into the breastmilk after SARS-CoV-2 infection. Cell Reports, 37(6), 109959.
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