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Flu glo

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Flu-Glo is a laboratory equipment product used for detecting influenza viruses. It functions as a diagnostic tool, providing a means to identify the presence of flu-related pathogens in samples.

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Lab products found in correlation

Sl 17, by Kowa (2 mentions) Sl d7, by Topcon (1 mentions) Proparacaine hydrochloride 0.5, by Alcon (1 mentions) Wild microscope mel53, by Leica (1 mentions)

6 protocols using flu glo

1

Comprehensive Canine Ophthalmic Examination

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At the initial visit and subsequent rechecks, each canine patient underwent a complete ophthalmic examination by a board-certified veterinary ophthalmologist or an ophthalmology resident, including: neuro-ophthalmic assessment (menace response, dazzle reflex, pupillary light reflexes, palpebral reflex), slit lamp biomicroscopy (SL-17; Kowa Company, Ltd., Tokyo, Japan), indirect ophthalmoscopy (Keeler Vantage; Keeler Instruments, Inc., Broomall, PA, USA), rebound tonometry (TonoVet; Icare Finland Oy, Espoo, Finland), Schirmer tear test-1 (STT-1; Eye Care Product Manufacturing LLC, Tucson, AZ, USA), and fluorescein staining (Flu-Glo, Akorn, Inc., Buffalo Grove, IL, USA).
Mauer A.N., Allbaugh R.A., Kreuder A.J, & Sebbag L. (2022). Impact of multi-drug resistance on clinical outcomes of dogs with corneal ulcers infected with Staphylococcus pseudintermedius. Frontiers in Veterinary Science, 9, 1083294.
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2

Ocular Health Assessment in Beagle Dogs

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Seven healthy female Beagle dogs (9–12 months, 10–15 kg) were purchased from Covance Laboratory (Cumberland, VA) and housed in a research facility at the University of Missouri. Prior to the onset of the study all dogs underwent a complete ophthalmic examination by a board certified veterinary ophthalmologist (EAG) which included slit lamp biomicroscopy (SL-15 Kowa Company, Ltd, Tokyo, Japan), indirect ophthalmoscopy (Wireless indirect ophthalmoscope, Keeler Instruments Inc., Broomall, PA, USA and pan retinal 2.2 indirect lens, Volk Optical Inc., Mentor, OH, USA), Schirmer tear test I (Schering-Plough Animal Health, Union, NJ, USA), tonometry (Tono-Pen Vet, Dan Scott and Associates, Westerville, OH, USA) and fluorescein staining (Flu-Glo, Akorn, Inc., Buffalo Grove, IL, USA). All dogs were determined to be free of ocular disease. The dogs received daily socialization and all other husbandry needs including a diurnal 12 hour light cycle. All studies were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the University of Missouri Institutional Animal Care and Use Committee.
Gronkiewicz K., Giuliano E., Kuroki K., Bunyak F., Sharma A., Teixeira L., Hamm C, & Mohan R. (2015). Development of a novel in vivo corneal fibrosis model in the dog. Experimental eye research, 143, 75-88.
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3

Comprehensive Ophthalmic Examination in Veterinary Patients

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A full ophthalmic examination was performed by two board-certified veterinary ophthalmologists (Asian College of Veterinary Ophthalmologists). Slit-lamp biomicroscopy (SL-D7; Topcon) and binocular indirect ophthalmoscopy (Vantage Indirect Ophthalmoscope; Keeler) were performed. When indicated, mydriasis was induced to examine the posterior segment of the eye with 1% tropicamide. A Schirmer tear test I (Schering-Plough Animal Health), fluorescein staining (Flu-Glo; Akorn Pharmaceuticals) and intraocular pressure measurements using a rebound tonometer (Tonovet; Icare Finland Oy) were performed in both eyes of all patients.
Park J., Kang S., Lee E, & Seo K. (2023). Prevalence of feline ophthalmic disorders in South Korea: a retrospective study (2009–2021). Journal of Feline Medicine and Surgery, 25(2), 1098612X231151478.
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4

Corneal Alkali Wound Model in Rabbits

Cited 1 time
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Using an established corneal wound model, corneal alkali wounding was induced in the left eye of each rabbit [35 (link)]. Briefly, after initial clinical examinations and extraocular imaging, rabbits were anesthetized by intramuscular injection of ketamine hydrochloride 50mg/kg (MWI, Boise, ID) and xylazine hydrochloride 10mg/kg (Akorn, Lake Forest IL). Proparacaine hydrochloride 0.5% (Alcon, Fort Worth, TX) was topically applied to the cornea and a wire eyelid speculum was placed. A 7mm-diameter filter paper was soaked in 0.5N sodium hydroxide (NaOH) solution and then applied onto the axial cornea for 30 seconds while visualized under a surgical microscope (Leica Wild Microscope MEL53; Leica, Wetzlar, Germany). Following removal of the filter paper, the wounded cornea was immediately and copiously rinsed with sterile balanced salt solution (BSS) to remove residual alkali solution. Fluorescein stain (Flu-Glo, Akorn, Inc., Buffalo Grove, IL, USA) was applied to verify corneal burns.
Fuchs A.A., Balne P.K., Giuliano E.A., Sinha N.R, & Mohan R.R. (2022). Evaluation of a novel combination of TRAM-34 and ascorbic acid for the treatment of corneal fibrosis in vivo. PLoS ONE, 17(1), e0262046.
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5

Healthy Beagle Dogs for Ophthalmic Research

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Six beagle dogs (1.5–2.0 years, 7.7–10.1 kg) were used in the study. The gender and neuter status was the same for all subjects (female spayed), as sex hormones are known to be key regulators of vascular tone in various organs, including the eye (Schmidl et al., 2015 (link)). All dogs were confirmed to be healthy based on complete physical and ophthalmic examinations, including slit-lamp biomicroscopy (SL-17; Kowa Company, Ltd., Tokyo, Japan), indirect funduscopy (Keeler Vantage; Keeler Instruments, Inc., Broomall, PA, USA), rebound tonometry (TonoVet; Icare Finland Oy, Espoo, Finland), Schirmer tear test-1 (STT-1; Eye Care Product Manufacturing LLC, Tucson, AZ, USA), and fluorescein staining (Flu-Glo, Akorn, Inc., Buffalo Grove, IL, USA). The dogs were group-housed in kennels with ambient temperature maintained at 18–24°C and lights automatically turned on/off at 06:00/18:00. The study was approved by the Institutional Animal Care and Use Committee of Iowa State University (log # 2-18-8704-K) and adhered to the Association for Research in Vision and Ophthalmology (ARVO) statement for the Use of Animals in Ophthalmic and Vision Research.
Sebbag L., Allbaugh R.A., Weaver A., Seo Y.J, & Mochel J.P. (2019). Histamine-Induced Conjunctivitis and Breakdown of Blood–Tear Barrier in Dogs: A Model for Ocular Pharmacology and Therapeutics. Frontiers in Pharmacology, 10, 752.
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6

Ocular Surface Assessment in Euthanized Cats

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Samples were collected from cats euthanized for reasons unrelated to the study, including chronic kidney disease, urethral obstruction, pulmonary hypertension, pulmonary carcinoma, cardiomyopathy, or rodenticide toxicity. Prior to euthanasia, each cat underwent slit-lamp biomicroscopy (Kowa SL-15 biomicroscope, Kowa Company Ltd., Tokyo, Japan) of the ocular adnexa and anterior segment, followed by fluorescein staining of the cornea and conjunctiva (Flu-Glo, Akorn Inc., Buffalo Grove, IL). Cats were excluded if any abnormality involving the eyelids or ocular surface was detected.
Sebbag L., Reilly C.M., Eid R, & Maggs D.J. (2016). Goblet cell density and distribution in cats with clinically and histologically normal conjunctiva. Veterinary ophthalmology, 19(Suppl 1), 38-43.
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