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Ready set go murine ifnγ elisa

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The Ready-SET-GO! Murine IFNγ ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kit designed to measure interferon-gamma (IFNγ) levels in murine samples.

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Lab products found in correlation

Heparan sulfate, by Merck Group (1 mentions) Opteia set, by BD (1 mentions)

Close spelling variants of this product

ELISA Ready-SET-Go (110 citations) Ready-SET-Go (98 citations) ELISAs (35 citations)

2 protocols using ready set go murine ifnγ elisa

1

Heparan Sulfate-Mediated IFNγ Binding Assay

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Five microgram of heparan sulfate (Sigma-Aldrich, St Louis, MO) was added into each well of 96-well plates at a volume of 100 µl/well for 2 hours at 37 °C. Wells were added with serially diluted supernatants of COS-7 cells transfected with pCMV-IFNγ or pCMV-IFNγ-HBDn. After incubation for 2 hours at room temperature, each well was washed with phosphate-buffered saline (PBS) −0.05% Tween 20, and proteins bound to heparan sulfate were detected by enzyme-linked immunosorbent assay (ELISA) using antimouse IFNγ antibody (Ready-SET-GO! Murine IFNγ ELISA; eBioscience, San Diego, CA).
Ando M., Takahashi Y., Yamashita T., Fujimoto M., Nishikawa M., Watanabe Y, & Takakura Y. (2014). Prevention of adverse events of interferon γ gene therapy by gene delivery of interferon γ-heparin-binding domain fusion protein in mice. Molecular Therapy. Methods & Clinical Development, 1, 14023-.
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2

Quantifying Murine IFNγ and IL12 p40

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The culture medium and serum of mice were collected at indicated times after the transfection. The murine IFNγ concentration was determined by Murine IFNγ ELISA kits (Ready-SET-GO! Murine IFNγ ELISA; eBioscience). The murine IL12 p40 concentration was determined using a murine IL 12 p40 ELISA kit (OptEIATM sets; Pharmingen, San Diego, CA).
Ando M., Takahashi Y., Yamashita T., Fujimoto M., Nishikawa M., Watanabe Y, & Takakura Y. (2014). Prevention of adverse events of interferon γ gene therapy by gene delivery of interferon γ-heparin-binding domain fusion protein in mice. Molecular Therapy. Methods & Clinical Development, 1, 14023-.
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