E coli er2566 competent cells

Manufactured by New England Biolabs

E. coli ER2566 competent cells are a strain of Escherichia coli bacteria that have been made competent, or able to take up and incorporate foreign DNA. They are commonly used in molecular biology and genetic engineering experiments as a host for recombinant DNA.

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Lab products found in correlation

Vent dna polymerase, by New England Biolabs (2 mentions) Nadph, by AppliChem (2 mentions) Benzamidine sepharose, by GE Healthcare (2 mentions) Gsh sepharose4 fast flow, by GE Healthcare (2 mentions) Sephacryls 200 resin, by Cytiva (2 mentions) N methyl mercaptoacetamide, by Merck Group (2 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (2 mentions) T4 dna ligase, by New England Biolabs (2 mentions) Quickchange site directed mutagenesis kit, by Agilent Technologies (2 mentions) Hydrogen peroxide, by Thermo Fisher Scientific (1 mentions) Cary 50 uv vis spectrophotometer, by Agilent Technologies (1 mentions) Chitin agarose beads, by New England Biolabs (1 mentions) Hydrogen peroxide 30 solution, by Thermo Fisher Scientific (1 mentions) L selenocystine, by Thermo Fisher Scientific (1 mentions)

2 protocols using e coli er2566 competent cells

Constructing PfTR Mutants via Mutagenic PCR

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All mutagenic polymerase chain reaction (PCR)
primers utilized in the construction of PfTR mutants were purchased
from Integrated DNA Technologies (Coralville, IA). The Quickchange
site-directed mutagenesis kit was purchased from Agilent Technologies
(Santa Clara, CA). Vent DNA polymerase, NdeI, SapI, T4 DNA ligase, E. coli ER2566 competent cells, and chitin-agarose beads
were purchased from New England Biolabs (Ipswich, MA). GSH-sepharose
4 Fast Flow, benzamidine sepharose, and 2′-4′-ADP-sepharose
resins were obtained from GE Healthcare (Waukesha, WI). Sephacryl
S-200 resin was purchased from Amersham Pharmacia (Uppsala, Sweden).
NADPH was obtained from AppliChem (Darmstadt, Germany). N-(Methyl)mercaptoacetamide, βME, and dl-α-lipoic
were from Sigma-Aldrich (St. Louis, MO). Bovine thrombin was purchased
from BioPharm Laboratories (Bluffdale, UT). l-Selenocystine
was from Acros Organics (Morris Plains, NJ). Hydrogen peroxide (30%
solution) and EDTA were purchased from Fisher Scientific (Fair Lawn,
NJ). All enzyme kinetic assays were performed on a Cary50 UV–vis
spectrophotometer (Cary, Walnut Creek, CA) and were conducted at
room temperature.

Snider G.W., Dustin C.M., Ruggles E.L, & Hondal R.J. (2014). A Mechanistic Investigation of the C-Terminal Redox Motif of Thioredoxin Reductase from Plasmodium falciparum. Biochemistry, 53(3), 601-609.

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Construction and Characterization of PfTR Mutants

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All mutagenic PCR primers utilized in the construction of PfTR mutants were purchased from Integrated DNA Technologies (Coralville, Iowa). The Quickchange site-directed mutagenesis kit was purchased from Agilent Technologies (Santa Clara, California). Vent DNA polymerase, NdeI, SapI, T4 DNA ligase, E. coli ER2566 competent cells, and chitin-agarose beads were purchased from New England Biolabs (Ipswich, MA). GSH-sepharose 4 Fast Flow, benzamidine sepharose, and 2′-4′-ADP-sepharose resins were obtained from GE Healthcare (Waukesha, WI). Sephacryl S-200 resin was purchased from Amersham Pharmacia (Uppsala, Sweden). NADPH was obtained from AppliChem (Darmstadt, Germany). N-(methyl)mercaptoacetamide, βME, and DL-α-lipoic were from Sigma-Aldrich (St. Louis, MO). Bovine thrombin was purchased from BioPharm Laboratories (Bluffdale, Utah). l-selenocystine was from Acros Organics (Morris Plains, New Jersey). Hydrogen peroxide (30% solution) and EDTA were purchased from Fisher Scientific (Fair Lawn, NJ). All enzyme kinetic assays were performed on a Cary50 UV-Vis spectrophotometer (Walnut Creek, CA) and were conducted at room temperature.

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