Safety multifly needle 21g

Manufactured by Sarstedt

Sourced in Germany

The Safety-Multifly®- Needle 21G is a single-use blood collection needle designed for use with vacuum blood collection systems. It features a safety shield to help protect users from accidental needle sticks.

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Lab products found in correlation

S monovettes k3 edta, by Sarstedt (2 mentions) S monovette, by Sarstedt (1 mentions) Monovette tube, by Sarstedt (1 mentions)

Close spelling variants of this product

Safety-Multifly Needle Safety-Multifly Multifly needles

4 protocols using safety multifly needle 21g

Blood Analysis of COVID-19 Patients

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3 mL whole blood from healthy donors, intensive-care COVID-19 patients and from patients who had recovered (convalescent donors) were collected in S-Monovettes EDTA K3 (Sarstedt, Nürnbrecht, Germany) by venipuncture using a sterile disposable Safety-Multifly®- Needle 21G (Sarstedt). Determination of red blood cells, hemoglobin content and hematocrit were performed on a Sysmex XN 1000 (Sysmex Deutschland GmbH, Norderstedt, Germany). Measurement of pH value and blood gas analyzing were carried out with an ABL800 Flex (Radiometer Medical ApS, Brønshø, Denmark).
Plasma was collected for measurement of iron metabolism parameters (performed in the Institute of Clinical Chemistry and Laboratory Medicine, Dresden).

Kronstein-Wiedemann R., Stadtmüller M., Traikov S., Georgi M., Teichert M., Yosef H., Wallenborn J., Karl A., Schütze K., Wagner M., El-Armouche A, & Tonn T. (2022). SARS-CoV-2 Infects Red Blood Cell Progenitors and Dysregulates Hemoglobin and Iron Metabolism. Stem Cell Reviews and Reports, 18(5), 1809-1821.

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Whole Blood Collection and Analysis

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Three milliliters whole blood from healthy donors were collected in S-Monovettes EDTA K3 (Sarstedt, Nürnbrecht, Germany) by venipuncture using sterile disposable Safety-Multifly®- Needle 21G (Sarstedt). Determination of hemoglobin contents and hematocrit values were performed with Sysmex XN 1000 (Sysmex Deutschland GmbH, Norderstedt, Germany).

Kronstein-Wiedemann R., Blecher S., Teichert M., Schmidt L., Thiel J., Müller M.M., Lausen J., Schäfer R, & Tonn T. (2023). Novel evidence that the ABO blood group shapes erythropoiesis and results in higher hematocrit for blood group B carriers. Leukemia, 37(5), 1126-1137.

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Pooled Normal Plasma Preparation

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Blood was collected in Monovette tubes (S-Monovette, 5 mL; sodium citrate, 0.106 mol/L; Sarstedt) from 10 healthy individuals (8 female; 2 male) after informed, written consent in 2017. The venepuncture was performed with a Safety-Multifly-Needle 21G (Sarstedt), and the first tube was discarded. The tubes were centrifuged (2,000g, 15 minutes, RT) within 1 hour, followed by transfer of the top plasma fraction to new tubes, leaving 0.5 cm plasma above the buffy coat. The plasmas were subjected to a second centrifugation (2,000g, 15 minutes, RT) and pooled together to constitute pooled normal plasma (PNP), which was aliquoted and stored at −80°C.

Rognes I.N., Hellum M., Ottestad W., Bache K.G., Eken T, & Henriksson C.E. (2021). Extracellular vesicle–associated procoagulant activity is highest the first 3 hours after trauma and thereafter declines substantially: A prospective observational pilot study. The Journal of Trauma and Acute Care Surgery, 91(4), 681-691.

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Thrombin Generation Assay Protocol

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Blood was collected in Monovette tubes (0.106 M citrate, 5 mL, Sarstedt, Nümbrecht, Germany) with and without manually prefilled CTI (18.3 μg/mL final concentration). The venipuncture was performed with a Safety-Multifly®-Needle 21G (Sarstedt, Nümbrecht, Germany), and the first tube was discarded. After written informed consent, eight healthy volunteers donated blood to four tubes with CTI and four tubes without CTI. The tubes were centrifuged (2,000 g, 15 min, RT) and pooled into two separate plasmas (+/- CTI) which were subjected to another centrifugation at 2,000 g, 15 min, RT. The PNP (+/- CTI) was aliquoted and stored at -80°C. At the day of use, the PNP (+/- CTI) was thawed at RT, and incubated (15 min at 37°) with anti-human TFPI abs (100 μg/mL final conc.) or an equal volume of TBSA before addition to the wells. The concentration of anti-TFPI abs was chosen based on initial in vitro thrombin generation experiments, where 100 μg/mL of anti-TFPI abs gave the highest sensitivity for detection of MVs obtained from whole blood stimulated with Neisseria meningitidis bacteria.

Hellum M., Franco-Lie I., Øvstebø R., Hauge T, & Henriksson C.E. (2017). The effect of corn trypsin inhibitor, anti-tissue factor pathway inhibitor antibodies and phospholipids on microvesicle-associated thrombin generation in patients with pancreatic cancer and healthy controls. PLoS ONE, 12(9), e0184579.

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