Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA). Streptozotocin (STZ) was obtained from Enzo Life Science (USA). Fatty acid-free bovine serum albumin fraction V, glycerol assay reagent, isoproterenol, penicillin-streptomycin, type-II collagenase, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES) were provided from Sigma (USA). Dimethyl sulfoxide and 3-isobutyl-1-methylxanthine (IBMX) were purchased from Fluka Chemical Co. Indomethacin and human insulin were kindly provided by EXIR Company (Iran).
4 2 hydroxyethyl piperazine 1 ethanesulfonic acid sodium salt hepes
Manufactured by Merck Group
Sourced in United States
4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES) is a common buffer compound used in biological research and cell culture applications. It maintains a stable pH range, typically between 6.8 and 8.2, and helps to maintain the physiological environment for cells and biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Isoproterenol, by Merck Group (2 mentions)
Glycerol assay reagent, by Merck Group (2 mentions)
Fatty acid free bovine serum albumin fraction 5, by Merck Group (2 mentions)
Collagenase type 2, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Streptozotocin stz, by Enzo Life Sciences (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide, by Merck Group (1 mentions)
3 isobutyl 1 methylxanthine, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
High glucose dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
2 protocols using 4 2 hydroxyethyl piperazine 1 ethanesulfonic acid sodium salt hepes
1
Adipogenesis Induction by STZ and Insulin
2
Isolation and Characterization of Human Adipocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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High glucose Dulbecco's Modified Eagles Medium (DMEM), fetal bovine serum (FBS), and trypsin were purchased from Gibco (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), fatty acid-free bovine serum albumin fraction V, glycerol assay reagent, isoproterenol (ISO), human adiponectin enzyme-linked immunosorbent assay (ELISA) kit, penicillin-streptomycin solution, type-II collagenase, 3-isobutyl-1-methylxanthine, 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Human C-peptide was purchased from Bachem (Bubendorf, Switzerland). Human insulin and indomethacin were kindly provided by Exir Company, Iran. Fluorescein isothiocyanate-conjugated antibodies against CD34, CD44, CD45, and CD105 were bought from AbD Serotec (Raleigh, NC, USA).
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