Dextran t70

Manufactured by Carl Roth

Sourced in Germany

Dextran T70 is a water-soluble polysaccharide derived from the bacterium Leuconostoc mesenteroides. It has an average molecular weight of approximately 70,000 Daltons. Dextran T70 is commonly used as a molecular weight standard, a volume expander in medical applications, and a component in cell culture media.

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4 protocols using dextran t70

Analysis of Glycation and Oxidation Effects

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Human serum albumin (HSA), methylglyoxal (MG), D-glucose, Histopaque, sodium citrate, 3,3′,5,5′-tetramethylbenzidine (TMB), phorbol 12-myristate 13-acetate (PMA), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), celestine blue B (CB), 4-chloro-1-naphtol (4-CN), Coomassie G-250, o-dianisidine, hydrogen peroxide (H₂O₂), horseradish peroxidase (HRP), luminol (Lum), lucigenin (Luc), Triton X-100 and all salts and solvents for the preparation of solutions were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide, methylene bis-acrylamide, tetramethylethylenediamine, ammonium persulphate, Tris and glycine were purchased from Panreac-AppliChem (Darmstad, Germany). Krebs-Ringer buffer solution was purchased from Merck (Kenilworth, NJ, USA). Anti-CD11b-PE, anti-CD63-APC, anti-CD45-FITC antibodies, non-fat dry milk (blotting grade blocker) and HRP-labeled anti-mouse IgG, nitrocellulose membrane were purchased from Bio-Rad (Hercules, CA, USA). Dextran T70 was purchased from Roth (Karlsruhe, Germany). May Grünwald’s Eosin–Methylene Blue solution and Romanowski Azur Eozin stain were purchased from ECOlab (Moscow, Russia).

Panasenko O.M., Ivanov V.A., Mikhalchik E.V., Gorudko I.V., Grigorieva D.V., Basyreva L.Y., Shmeleva E.V., Gusev S.A., Kostevich V.A., Gorbunov N.P, & Sokolov A.V. (2022). Methylglyoxal-Modified Human Serum Albumin Binds to Leukocyte Myeloperoxidase and Inhibits its Enzymatic Activity. Antioxidants, 11(11), 2263.

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Synthesis of Dextran-Coated Iron Oxide Nanoparticles

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UL-D nanoparticles were synthesized by classical co-precipitation of iron oxide in an alkaline solution in the presence of dextran, a high molecular weight polymer of d-glucose.20 ,21 (link) Six mmol iron (II) chloride tetrahydrate (FeCl₂·4H₂O) and 27.8 mmol iron (III) chloride hexahydrate (FeCl₃·6H₂O) (VWR International GmbH, Darmstadt, Germany) were dissolved in 150 mL H₂O with 12.6 g dextran T70 (MW 70,000; Carl Roth GmbH & Co., KG, Karlsruhe, Germany). Iron hydroxide precipitated after dropwise addition of 150 mL 7.5% base ammonia (NH₃) at room temperature. After heating the solution for 30 minutes to 60 minutes at 70°C, the iron hydroxide was reduced to iron oxide and Fe₃O₄ and dextran enclosed the particles. After synthesis, the particles were centrifuged for size separation and dialyzed against H₂O. Several steps of separation and purification were performed to concentrate the nanoparticles.

Lindemann A., Lüdtke-Buzug K., Fräderich B.M., Gräfe K., Pries R, & Wollenberg B. (2014). Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells. International Journal of Nanomedicine, 9, 5025-5040.

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Comprehensive Reagents and Protocols

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CaCl₂, ≥93.0%; Na₂CO₃, ≥99.0%; Histopaque 1.077, 1.119; Krebs–Ringer solution; Folin–Ciocalteu reagent; human serum albumin fraction V (HSA); methylglyoxal; lucigenin; superoxide dismutase (SOD); horseradish peroxidase (HRP); sodium azid (NaN₃); scopoletin; tri sodium citrate; genistein; wortmannin; diphenyleneiodonium chloride (DPI); ethylenedinitrilotetraacetatic acid (EDTA); Tris; 3-((3-cholamidopropyl) dimethylammonio)-1-propanesufonate (CHAPS); nonyl phenoxypolyethoxylethanol (NP40); N,N,N′,N′-tetramethyl ethylenediamine (TEMED); ammonium persulfate; glycerol; sodium dodecyl sulfate (SDS); thiourea; acrylamide; and bisacrilamide were all purchased from Sigma-Aldrich (St. Louis, MO, USA); glycine was purchased from ApliChem GmbH (Darmstadt, Germany); gelatin from Fluka (Seelze, Germany) (; dextran T70 from Roth, Germany; USP-grade urea from Amresco (Solon, OH, USA); 2,5-dihydroxybenzoic acid from Bruker Daltonics (Billerica, MA, USA); cyanines from Lumiprob (Hunt Valley, ML, USA); ampholines, DIGE standards, dithiothreitol (DTT) from BioRad (Hercules, CA, USA); and NaH₂PO₄∙2H₂O, propanol-2, CuSO₄·5H₂O, NaCl, Eur Ph grade CaCl₂·2H₂O and sodium citrate were purchased from “Chimmed”(Moscow, Russia).

Mikhalchik E.V., Ivanov V.A., Borodina I.V., Pobeguts O.V., Smirnov I.P., Gorudko I.V., Grigorieva D.V., Boychenko O.P., Moskalets A.P., Klinov D.V., Panasenko O.M., Filatova L.Y., Kirzhanova E.A, & Balabushevich N.G. (2022). Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin. International Journal of Molecular Sciences, 23(14), 7840.

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Nanoparticle Characterization and Cytotoxicity

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Nanoparticles for intravascular applications: physicochemical characterization & cytotoxicity testing Research Article Dextran T70 was from Roth (Karlsruhe, Germany), or from Amersham Pharmacia Biotech (Freiburg, Germany), and dextran T40 from PharmaCosmos (Holbaek, Denmark). Carboxymethyl-dextran sodium salt (CM-Dextran) was purchased from Sigma Aldrich (Saint Quentin Fallavier, France) and diethylaminoethyl-dextran 20 (DEAE-dextran) from TdB Consultancy (Uppsala, Sweden). Low molecular weight Fucoidan (3-8 kDa, Fucoidan Ascophyscient ® ) was from Algues et Mer (Ouessant, France). IBCA (isobutylcyanoacrylate, Glue 368) was from Orapi (Saint-Vulbas, France). Bovine serum albumin (BSA) and iron (II) chloride tetrahydrate were from Merck, Darmstadt, Germany. Lauric acid, epichlorohydrin and dextranT6 (Mw = 6 kDa) were from Sigma Aldrich, Munich, Germany. Ceric (IV) ammonium nitrate and tri-sodium citrate dihydrate were purchased from Fluka (Saint Quentin Fallavier, France). NaOH, HCl (25%), NH 3 (25%) and nitric acid (65%w/w) were from Roth. Iron (III) chloride hexahydrate was purchased from Sigma-Aldrich or from Roth. All compounds used were of pharmaceutical (Ph. Eur) or highly pure (≥99%) grade and were used without any further purification.

Matuszak J., Baumgartner J., Zaloga J., Juenet M., da Silva A.E., Franke D., Almer G., Texier I., Faivre D., Metselaar J.M., Navarro F.P., Chauvierre C., Prassl R., Dézsi L., Urbanics R., Alexiou C., Mangge H., Szebeni J., Letourneur D, & Cicha I. (2016). Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing. Nanomedicine (London, England), 11(6).

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