Conjugated Abs anti-mouse CD45 (clone 30-F11), CD3ε (500A2 or 17A2), CD19 (6D5), CD11b (M1/70), NK1.1 (PK136), NKp46 (29A1.4), CD90.2 (30-H12), ST2 (DIH9 and RMST2-2), ICOS (C398.4A), CD25 (PC61), c-kit (2B8), Sca-1 (D7), IL-17RB (752101), CCR6 (29-2L17), IFN-γ (XMG1.2), IL-5 (TRFK5), IL-13 (eBio13A), IL-22 (Poly5164), IL-17A (TC11-18H10.1), RORγt (Q31-378), Eomes (Dan11mag) and CD16/32-Fc blocking (93); Abs anti-human CD45 (HI30), CD3 (UCHT1), CD19 (HIB19), CD14 (M5E2), CD127 (A019D5), CD9 (M-L13), CD56 (HCD56), CRTH2 (BM16), c-kit (104D2), NKp44 (P44-8), RORγt (Q21-559) and human Fc blocking; anti-human/mouse GATA-3 (TWAJ and 16E10A23) and T-bet (O4-46) were purchased from Biolegend, eBioscience, BD Biosciences or R&D Systems. Cytokines and transcription factors were stained using the FoxP3 staining buffer set (eBioscience) according to the manufacturer’s instructions. Fixable viability dyes eFluor 780 and eFluor 506 (eBioscience) was used to exclude dead cells. Samples were acquired on a LSR Fortessa (BD Biosciences) using FACS DiVa and analyzed using FlowJo (Tree Star).
Anti human mouse gata 3 twaj and 16e10a23
Manufactured by BioLegend
Anti-human/mouse GATA-3 (TWAJ and 16E10A23) is a flow cytometry reagent designed to detect the transcription factor GATA-3 in human and mouse cells. GATA-3 is a critical regulator of T-helper 2 cell differentiation and is involved in the development and function of various immune cell types.
Automatically generated - may contain errors
Lab products found in correlation
Rorγt q21 559, by BioLegend (2 mentions)
Efluor 780, by Thermo Fisher Scientific (2 mentions)
Efluor 506, by Thermo Fisher Scientific (2 mentions)
Foxp3 staining buffer set, by Thermo Fisher Scientific (2 mentions)
Lsrfortessa, by BD (2 mentions)
T bet o4 46, by BioLegend (2 mentions)
Facs diva, by Tree Star (1 mentions)
2 protocols using anti human mouse gata 3 twaj and 16e10a23
1
Comprehensive Immune Cell Profiling
2
Comprehensive Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Conjugated Abs anti-mouse CD45 (clone 30-F11), CD3ε (500A2 or 17A2), CD19 (6D5), CD11b (M1/70), NK1.1 (PK136), NKp46 (29A1.4), CD90.2 (30-H12), ST2 (DIH9 and RMST2-2), ICOS (C398.4A), CD25 (PC61), c-kit (2B8), Sca-1 (D7), IL-17RB (752101), CCR6 (29-2L17), IFN-γ (XMG1.2), IL-5 (TRFK5), IL-13 (eBio13A), IL-22 (Poly5164), IL-17A (TC11-18H10.1), RORγt (Q31-378), Eomes (Dan11mag), and CD16/32-Fc blocking (93); Abs anti-human CD45 (HI30), CD3 (UCHT1), CD19 (HIB19), CD14 (M5E2), CD127 (A019D5), CD9 (M-L13), CD56 (HCD56), CRTH2 (BM16), c-kit (104D2), NKp44 (P44-8), RORγt (Q21-559) and human Fc blocking; anti-human/mouse GATA-3 (TWAJ and 16E10A23) and T-bet (O4-46) were purchased from Biolegend, eBioscience, BD Biosciences, or R&D Systems. Cytokines and transcription factors were stained using the Foxp3 staining buffer set (eBioscience) according to the manufacturer’s instructions. Fixable viability dyes eFluor 780 and eFluor 506 (eBioscience) were used to exclude dead cells. Samples were acquired on an LSR Fortessa (BD Biosciences) using FACSDiva and analyzed using FlowJo (Tree Star).
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