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Igg1 alexa fluor 555

Manufactured by Thermo Fisher Scientific
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IgG1 Alexa Fluor 555 is a fluorescently labeled antibody used for detection and visualization in various biological applications. It consists of an IgG1 antibody conjugated to the Alexa Fluor 555 dye, which has an excitation maximum at 555 nm and an emission maximum at 565 nm.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (4 mentions) Krt19, by Agilent Technologies (2 mentions) Igg3 alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Krt14, by Abcam (2 mentions)

Close spelling variants of this product

Alexa Fluor® 555 goat anti-mouse IgG1 antibody Goat anti-mouse IgG1 Alexa Fluor 555 conjugate Alexa Fluor 555-goat anti-mouse IgG1 Alexa Fluor 555 anti-Mouse IgG1 Alexa Fluor 555 IgG1 for MHCIIa Alexa Fluor 555 Alexa 555 Alexa Fluors

2 protocols using igg1 alexa fluor 555

1

Immunostaining and Quantification of Adherent Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Array-bound and well-bound cells were fixed in 2% PFA for 15 minutes at RT following respective treatments. Cells were then permeabilized with .3% Triton X-100 for 25 minutes at RT. Array-bound cell primary antibody staining was performed with KRT14 (Abcam, 1:200), KRT19 (Dako, 1:200), and DAPI (ThermoFisher, 1:10,000). Secondary antibody staining was performed with IgG3 Alexa Fluor 488 (ThermoFisher, 1:200), and IgG1 Alexa Fluor 555 (ThermoFisher, 1:200). Only DAPI and EdU detection was performed on well-bound cells, with the exception of Figure 2B. Well plates were imaged on the GE InCell 6000 platform, and image analysis and cell count quantification were performed on the GE InCell Analyzer software package. Size gating of nuclei was used to exclude apoptotic cells, and EdU positivity was determined as nuclei having a mean fluorescent intensity above an experimentally consistent threshold (this threshold was defined using single cell parametric analysis plotting total DAPI intensity against mean EdU intensity). All fluorescent imaging studies were performed at consistent intensity and gain settings across experiments.
Watson S.S., Dane M., Chin K., Tatarova Z., Liu M., Liby T., Thompson W., Smith R., Nederlof M., Bucher E., Kilburn D., Whitman M., Sudar D., Mills G.B., Heiser L.M., Jonas O., Gray J.W, & Korkola J.E. (2018). Microenvironment-Mediated Mechanisms of Resistance to HER2 Inhibitors Differ between HER2+ Breast Cancer Subtypes. Cell systems, 6(3), 329-342.e6.
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2

Immunostaining and Quantification of Adherent Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Array-bound and well-bound cells were fixed in 2% PFA for 15 minutes at RT following respective treatments. Cells were then permeabilized with .3% Triton X-100 for 25 minutes at RT. Array-bound cell primary antibody staining was performed with KRT14 (Abcam, 1:200), KRT19 (Dako, 1:200), and DAPI (ThermoFisher, 1:10,000). Secondary antibody staining was performed with IgG3 Alexa Fluor 488 (ThermoFisher, 1:200), and IgG1 Alexa Fluor 555 (ThermoFisher, 1:200). Only DAPI and EdU detection was performed on well-bound cells, with the exception of Figure 2B. Well plates were imaged on the GE InCell 6000 platform, and image analysis and cell count quantification were performed on the GE InCell Analyzer software package. Size gating of nuclei was used to exclude apoptotic cells, and EdU positivity was determined as nuclei having a mean fluorescent intensity above an experimentally consistent threshold (this threshold was defined using single cell parametric analysis plotting total DAPI intensity against mean EdU intensity). All fluorescent imaging studies were performed at consistent intensity and gain settings across experiments.
Watson S.S., Dane M., Chin K., Tatarova Z., Liu M., Liby T., Thompson W., Smith R., Nederlof M., Bucher E., Kilburn D., Whitman M., Sudar D., Mills G.B., Heiser L.M., Jonas O., Gray J.W, & Korkola J.E. (2018). Microenvironment-Mediated Mechanisms of Resistance to HER2 Inhibitors Differ between HER2+ Breast Cancer Subtypes. Cell systems, 6(3), 329-342.e6.
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