Diamide

Manufactured by Santa Cruz Biotechnology

Diamide is a chemical reagent used in biochemical research. Its core function is to induce the formation of disulfide bonds between cysteine residues in proteins. This process is often utilized to study protein structure and stability.

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Lab products found in correlation

3 protocols using diamide

Cellular redox regulation assays

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5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB) was purchased from Merck. NADPH, DL-methionine sulfoxide and oxidized glutathione (GSSG) were purchased from Sigma whereas diamide was purchased from Santa Cruz Biotechnology. Growth media components were either bought from Merck (yeast extract, peptone from casein, sodium chloride), from Sigma (proteose peptone, fetal bovine serum). The TrxR inhibitors auranofin and aurothioglucose were purchased from Sigma.

Leitsch D., Mbouaka A.L., Köhsler M., Müller N, & Walochnik J. (2021). An unusual thioredoxin system in the facultative parasite Acanthamoeba castellanii. Cellular and Molecular Life Sciences, 78(7), 3673-3689.

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Investigating G6PD and SIRT2 in Glioma

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Antibodies against G6PD (Abcam), SIRT2 (Epitomics) were purchased commercially. MNNG (Tokyo Chemical Industry, TCI), temozolomide (TMZ, TCI), H₂O₂ (Sigma), diamide (Santa Cruz) were purchased commercially. Catalase expression plasmid was purchased from Addgene.

Ye H., Huang H., Cao F., Chen M., Zheng X, & Zhan R. (2016). HSPB1 Enhances SIRT2-Mediated G6PD Activation and Promotes Glioma Cell Proliferation. PLoS ONE, 11(10), e0164285.

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Pharmacological Agents for Cellular Assays

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The following pharmacological agents were used: bicuculline (ENZO Life Sciences; catalog no.: ALX-550-515; 50 μM), CGP37157 (Sigma–Aldrich; catalog no.: C8874; 10 μM), diamide (Santa Cruz Biotechnology; catalog no.: 10465-78-8; 0.5 mM), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) (Sigma–Aldrich; catalog no.: C2920; 5 μM), gabazine (SR 99531 hydrobromide; Biotrend; catalog no.: BN0507; 5 μM), NMDA (Biotrend; catalog no.: BN0385; 5, 10, or 20 μM), and Ru360 (Merck Millipore; catalog no.: 557440; 10 μM).

Hagenston A.M., Yan J., Bas-Orth C., Tan Y., Sekler I, & Bading H. (2021). Disrupted expression of mitochondrial NCLX sensitizes neuroglial networks to excitotoxic stimuli and renders synaptic activity toxic. The Journal of Biological Chemistry, 298(2), 101508.

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