Cotton-tipped swabs (Medi-Choice, Mechanicsville, Virginia) were used to collect buccal mucosa cells from 2 siblings with truncating variants in FLNC and from normal control subjects. Each cheek was rubbed with a slight rolling and scraping motion, and the resulting material was smeared on standard microscope slides. Immunostaining of buccal mucosa and myocardial tissue was performed as previously described (24 (link)), and is detailed in the Online Appendix . For immunofluorescence microscopy, antibodies included: mouse monoclonal antiplakoglobin (P8087, Sigma-Aldrich, St. Louis, Missouri), mouse monoclonal anti–connexin 43 (Cx43) (Millipore, Burlington, Massachusetts), mouse monoclonal anti–N-cadherin (Sigma-Aldrich), mouse monoclonal antidesmoplakin (Fitzgerald, Acton, Massachusetts), mouse monoclonal anti–synapse-associated protein 97SAP97 (Santa Cruz, Dallas, Texas), and rabbit polyclonal antiglycogen synthase kinase 3β (GSK3β) (Cell Signaling Technology, Danvers, Massachusetts). Immunostained preparations were analyzed by confocal microscopy (LSM-510, Zeiss, Oberkochen, Germany).
Mouse monoclonal anti synapse associated protein 97sap97
Manufactured by Santa Cruz Biotechnology
Mouse monoclonal anti–synapse-associated protein 97 (SAP97) is a laboratory reagent used for the detection and study of the SAP97 protein. SAP97 is a membrane-associated guanylate kinase that plays a role in the organization of synaptic protein complexes.
Automatically generated - may contain errors
Lab products found in correlation
Antiglycogen synthase kinase 3β gsk3β, by Cell Signaling Technology (2 mentions)
Mouse monoclonal antiplakoglobin p8087, by Merck Group (2 mentions)
Mouse monoclonal anti connexin 43 cx43, by Merck Group (2 mentions)
Mouse monoclonal anti n cadherin, by Merck Group (2 mentions)
Mouse monoclonal antidesmoplakin, by Biosynth (2 mentions)
Lsm 510, by Zeiss (2 mentions)
2 protocols using mouse monoclonal anti synapse associated protein 97sap97
1
Immunostaining of Buccal Mucosa Cells
2
Immunostaining of Buccal Mucosa Cells
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Cotton-tipped swabs (Medi-Choice, Mechanicsville, Virginia) were used to collect buccal mucosa cells from 2 siblings with truncating variants in FLNC and from normal control subjects. Each cheek was rubbed with a slight rolling and scraping motion, and the resulting material was smeared on standard microscope slides. Immunostaining of buccal mucosa and myocardial tissue was performed as previously described (24 (link)), and is detailed in the Online Appendix . For immunofluorescence microscopy, antibodies included: mouse monoclonal antiplakoglobin (P8087, Sigma-Aldrich, St. Louis, Missouri), mouse monoclonal anti–connexin 43 (Cx43) (Millipore, Burlington, Massachusetts), mouse monoclonal anti–N-cadherin (Sigma-Aldrich), mouse monoclonal antidesmoplakin (Fitzgerald, Acton, Massachusetts), mouse monoclonal anti–synapse-associated protein 97SAP97 (Santa Cruz, Dallas, Texas), and rabbit polyclonal antiglycogen synthase kinase 3β (GSK3β) (Cell Signaling Technology, Danvers, Massachusetts). Immunostained preparations were analyzed by confocal microscopy (LSM-510, Zeiss, Oberkochen, Germany).
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