Antibodies against AKT (#9272), phospho-AKT (Ser473, #9271), phospho-AKT (Thr308, #2965), S6K1 (#9202), phospho-S6K1 (Thr389, #9205), S6 ribosomal protein (#2217), phospho-S6 ribosomal protein (Ser235/236, #2211), 4E-BP1 (#9452), and phospho-4E-BP1 (Thr37/46, #9459) were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody against α-tubulin (T9026) was purchased from Sigma Aldrich (St. Louis, MO, USA). The antibody against puromycin (clone 12D10, MABE343) was purchased from Merck Millipore (Burlington, MA, USA).
Antibody against α tubulin t9026
Manufactured by Merck Group
Sourced in United States
Antibody against α-tubulin (T9026) is a research-use antibody that specifically binds to the α-tubulin protein, a component of the cytoskeleton in eukaryotic cells. This antibody can be used to detect and study the distribution and dynamics of α-tubulin in various cell types and applications.
Automatically generated - may contain errors
Lab products found in correlation
Phospho s6k t389, by Cell Signaling Technology (1 mentions)
Anti rabbit 7074, by Cell Signaling Technology (1 mentions)
Chemidoc mp imager, by Bio-Rad (1 mentions)
Trans blot turbo transfer system, by Bio-Rad (1 mentions)
Midi kit, by Bio-Rad (1 mentions)
Protease inhibitor p8340, by Merck Group (1 mentions)
Lc3a b, by Cell Signaling Technology (1 mentions)
2 protocols using antibody against α tubulin t9026
1
AKT and mTOR Pathway Antibody Protocol
2
Western Blot Analysis of Cellular Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the respective infections and/or treatments, cells were lysed with RIPA buffer composed with protease inhibitor (P8340) (Sigma), phosphatase inhibitors (P0044 and P5726) (Sigma), and PMSF (1 mM). After the electrophoresis process, proteins were transferred to a nitrocellulose membrane (Midi kit, Bio-Rad, Marnes-la-Coquette, France) using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were imaged using a ChemiDoc MP imager (Bio-Rad).
Antibodies against S6 (#2217, dilution 1:1,000), phospho-S6 (Ser235/236) (#4858, dilution 1:1,000), S6K (#2708, dilution 1:1,000), phospho-S6K(T389) (#9205, dilution 1:1,000), and LC3A/B (#12,741, dilution 1:1,000) were obtained from Cell Signaling Technology (Leiden, The Netherlands). Antibody against α-tubulin (T9026, dilution 1:5,000) was obtained from Sigma. The secondary antibodies anti-mouse (#7076, dilution 1:1,000) and anti-rabbit (#7074, dilution 1:1,000) were obtained from Cell Signaling Technology.
Antibodies against S6 (#2217, dilution 1:1,000), phospho-S6 (Ser235/236) (#4858, dilution 1:1,000), S6K (#2708, dilution 1:1,000), phospho-S6K(T389) (#9205, dilution 1:1,000), and LC3A/B (#12,741, dilution 1:1,000) were obtained from Cell Signaling Technology (Leiden, The Netherlands). Antibody against α-tubulin (T9026, dilution 1:5,000) was obtained from Sigma. The secondary antibodies anti-mouse (#7076, dilution 1:1,000) and anti-rabbit (#7074, dilution 1:1,000) were obtained from Cell Signaling Technology.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!