Deaprotinin

N-acetyl-cysteine (NAC), Ferric ammonium citrate (FAC), 2′, 7′-dichlorodihy-drofluorescein diacetate (H2DCF-DA), calcein-AM, Hoechst33258, 4′, 6-diamidino-2-phenylindol (DAPI), penicillin, streptomycin, leupeptin, pepstatin A, deaprotinin, phenylmethylsulfonylfluoride (PMSF), and 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) were purchased from Sigma (St. Louis, MO, USA). Primary antibodies against cytochrome c, bcl-2, bax, cleaved Caspase-3, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and beta-actin (β-actin) were purchased from Abcam (Cambridge, UK). AnnexinV–FITC/PI kit was obtained from KeyGen Biotech (Nanjing, China). Fetal bovine serum and alpha-modified Eagle’s medium (a-MEM) were purchased from Gibco (Waltham, MA, USA). Cell Mitochondria Isolation Kit, Enhanced Chemiluminescence detection kit, Western and immunoprecipitation (IP) Cell Lysis Kit, Bicinchoninic Acid Protein (BCA) Protein Assay Kit, and 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanineiodide (JC-1) were purchased from Beyotime (China). Cell Counting Kit-8 (CCK-8) assay kit was purchased from DojinDo (Japan).

Eight hours after the administration of QA or PBS, the right and left striatum were dissected and kept at −80 °C until tested. Samples were homogenized in 200 μL of protease inhibitor solution [NaCl 0,4 M; Tween 20 (Synth) 0.05%; bovine serum albumin (BSA) (Inlab Confiança) 0.5%; phenylmethylsulfonylfluoride (Calbiochem) 0.1 mM; benzethonium chloride (Vetec) 0.1 mM; EDTA 10 mM; 20 UI de aprotinin (Sigma), diluted in PBS]. Homogenates were centrifuged at 4 °C at 10,000 rpm, aliquoted, and stored at −80 °C until analysis. Brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and interleukin (IL)1β protein levels were assessed using ELISA kits (R&D Systems, Abingdon, UK) according to the manufacturer protocol. IL-2, IL-4, IL-6, interferon (IFN) γ, tumor necrosis factor alpha (TNF-α), IL-10, and IL-17A were assessed using Th1/Th2 kit (BD Pharmigen, CA, USA) by Cytometric Bead Array (CBA) method according to the protocol of the manufacturer. The results of the ELISA and CBA were expressed as pg/mg of total protein.