Scarb2

Manufactured by Abcam

Sourced in United States

SCARB2 is a protein-coding gene that provides instructions for producing the SCARB2 protein. This protein is involved in the transport and lysosomal targeting of certain enzymes. SCARB2 plays a role in the normal functioning of lysosomes, which are compartments within cells that break down and recycle various molecules.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using scarb2

Exosomal Protein Profile Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples of treated cells, exosomes and MVs fraction were lysed in RIPA buffer(Santa Cruz, USA) for 20 min on ice and cleared lysate was collected by centrifugation for protein separation on 10% SDS-PAGE gels(Bio-Rad, USA). The proteins were transferred onto Immobilon-FL PVDF Transfer Membrane(Millipore) and detected with appropriate primary antibodies at 4°C overnight, followed by incubation with HRP-conjugated anti-rabbit IgG or anti-mouse IgG (Southern Biotech) secondary antibodies. Membranes were exposed using an enhanced chemoluminescence(NcmECL Ultra) system(NCM Biotech, Suzhou, China) and band intensities were quantified by ImageJ software(NIH). Primary antibodies against CD63(BD Biosciences), CD9(Sigma, #SAB4503606), DAF(#31759), CVB3 VP1(Dako,#M706401-2), Annexin A1(#32934), Annexin A5(#8555), GM130(#12480), Calnexin(#2679) (all from Cell Signaling Technology, USA), CAR(#ab272711), Alix(#ab186429), SCARB2(#ab240186), Tyro3(#ab109231), GAPDH(#ab8245)(all from Abcam, USA), CAV16 VP1(GTX132338), CAV16 3AB(GTX132344), Zika Envelope(GTX133314), Zika Capsid(GTX133317)(All from GeneTex, Inc. Taiwan, China).

Fu Y, & Xiong S. (2023). Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism. PLOS Pathogens, 19(1), e1011090.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were lysed in the M-PER mammalian protein extraction reagent (Thermo, Waltham, MA, USA) containing halt protease inhibitor single-use cocktail (Thermo). The protein concentration was determined by the BCA reagents (Thermo). About 15 μg proteins were denatured and applied to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoresis products were transferred to a polyvinyl ideneﬂuoride (PVDF) membrane. The membranes were then incubated at room temperature with specific primary antibody. After a standard washing, membranes were incubated with horse radish peroxidase (HRP)-labelled secondary antibody. The assay developed using a chemiluminescent substrate. The primary antibodies used in this study included antibodies against β-actin, GFP-tag, Flag-tag, HA-tag, and Myc-tag from Cell Signalling Technology (Beverly, MA, USA), SCARB2 from Abcam (Cambridge, MA, USA), TRIB3 from OriGene (Wuxi, China) and EV-A71 VP1 from GeneTex (California, USA). The goat anti-rabbit and anti-mouse HRP-labelled antibodies were obtained from Cell Signalling Technology.

Wang H., Li K., Cui B., Yan H., Wu S., Wang K., Yang G., Jiang J, & Li Y. (2024). Tribbles pseudokinase 3 promotes enterovirus A71 infection via dual mechanisms. Emerging Microbes & Infections, 13(1), 2307514.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!