Ab175449

Pericontusive cortex (3 × 3 × 5 mm (width × depth × length) surrounding the edge of the lesion) proteins were extracted (n = 6), and protein levels of Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), NF-κB, NLRP3, apoptosis-associated speck-like protein (ASC), and caspase-1 were measured by western blotting. Protein concentrations from tissue lysates were measured using the bicinchoninic acid method (cat. no. PA115; Tiangen Biotech). Primary antibodies for western blotting were anti-Bax (1:1000, Abcam, ab32503), anti-Bcl-2 (1:1000, Abcam, ab59348), anti-NF-κB (1:1000, Cell Signaling Technology, 8242), anti-NLRP3 (1;500, Abcam, ab214185), anti-ASC (1:500, Abcam, ab175449), anti-caspase-1 (1:1000, Novusbio, NB100-56565), and anti-GAPDH (1:2000, Proteintech, 60004-1-lg). Following sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the protein from each sample was transferred to the polyvinylidene fluoride (PVDF) membrane. To make the primary antibodies bind to the secondary antibodies, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000, Zsgb-Bio) for 60 minutes at room temperature. Western blot results were analyzed using Image Lab Software (version 3.0).