Antibodies and reagents used in the study are listed as following: anti-Plk1 (Sigma-Aldrich, P5998, 1:10,000), anti-Flag (Sigma, F3165, 1:5000), anti-HA (Sigma, H9658, 1:5000), anti-G6PD (Protein-tech, 25413-1-AP, 1:1000), anti-G6PD (Abcam, ab993, 1:1000), anti-glutathione S-transferase (anti-GST; Protein-tech, 10000-0-AP, 1:5000), anti-phospho-H3Ser10 (Millipore, 06-570, 1:1000), anti-cyclin B1 (Protein-tech, 55004-1-AP, 1:1000), anti-phospho-threonine (Millipore, AB1607, 1:1000), anti-PGLS (Santa Cruz, SC-398833, 1:1000), anti-6PGD (Protein-tech, 14718-1-AP, 1:1000), anti-CDK2 (Protein-tech, 10122-1-AP, 1:1000), rabbit IgG (Protein-tech), mouse IgG (Santa Cruz), and anti-Actin (Protein-tech, 60008-1-Ig, 1:5000). NADP+, NADPH, GSH, G6P, 6PG, NAC, Dox, tetracycline, HU, propidium iodide, nocodazole, 4,6-diamidino-2-phenylindole (DAPI), casein, D2O, ribonucleosides, and deoxyribonucleosides were purchased from Sigma-Aldrich; R5P, nucleotides (AXP, GXP, CXP, and UXP, X = M, D, and T) were purchased from Sangon Biotech. BI2536 was purchased from Selleck Corporation, PP2A was from Millipore, and human recombinant Plk1 was from Sino biological company. [2-13C]-glucose and [U-13C]-glucose were from Cambridge Isotope Laboratories. γ-32P ATP was from China Isotope & Radiation Corporation.
Ribonucleosides
Manufactured by Merck Group
Ribonucleosides are organic compounds that serve as the fundamental building blocks of ribonucleic acid (RNA). They consist of a ribose sugar molecule covalently bonded to a nitrogenous base, which can be one of the four bases found in RNA: adenine, guanine, cytosine, or uracil. Ribonucleosides play a crucial role in various biological processes and are essential for the synthesis and function of RNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
Anti ha, by Merck Group (1 mentions)
Anti flag, by Merck Group (1 mentions)
Tetracycline, by Merck Group (1 mentions)
Anti actin, by Proteintech (1 mentions)
Anti plk1, by Merck Group (1 mentions)
Anti cdk2, by Proteintech (1 mentions)
Anti g6pd, by Proteintech (1 mentions)
Anti phospho h3ser10, by Merck Group (1 mentions)
Anti cyclin b1, by Proteintech (1 mentions)
Anti phospho threonine, by Merck Group (1 mentions)
2 13c glucose, by Cambridge Isotopes (1 mentions)
Anti g6pd, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Casein, by Merck Group (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
Nocodazole, by Merck Group (1 mentions)
U 13c glucose, by Cambridge Isotopes (1 mentions)
2 protocols using ribonucleosides
1
Plk1 Regulation of Pentose Phosphate Pathway
2
Functionalized Magnetic Nanoparticles for Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N-fluorenylmethoxycarbonyl
diphenylalanine (Fmoc-FF) and N-fluorenylmethoxycarbonyl
arginylglycylaspartic acid (Fmoc-RGD) peptides were purchased from
Bachem Co. (Bubendorf BL, Switzerland) and were used without further
purification. Magnetic nanoparticles (MNP) made of iron (purity of
99.7% or higher) and with diameters in the range of 60–80 nm
were purchased from SkySpring Nanomaterials, Inc. (Houston, TX, USA).
Polyethylene glycol with a molecular mass of 400 g mol–1 (PEG-400), glutaraldehyde (25% solution in water), mineral oil,
and sorbitan sesquioleate were all provided by Sigma-Aldrich (Burlington,
MA, USA). n-Hexane (minimum purity of 99%) was purchased
from Scharlab SL (Barcelona, Spain). Dulbecco’s modified Eagle’s
Medium (DMEM), penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL)
solution, and UltraGlutamine 1200 mM (U-Gln) were all purchased from
Lonza (Basel, Switzerland). Alpha modification of minimum essential
medium (α modification with sodium bicarbonate, ribonucleosides,
and deoxyribonucleosides withoutl -glutamine) was purchased
from Sigma-Aldrich. HyClone characterized fetal bovine serum (FBS)
was purchased from Cytiva (Marlborough, MA, USA).
diphenylalanine (Fmoc-FF) and N-fluorenylmethoxycarbonyl
arginylglycylaspartic acid (Fmoc-RGD) peptides were purchased from
Bachem Co. (Bubendorf BL, Switzerland) and were used without further
purification. Magnetic nanoparticles (MNP) made of iron (purity of
99.7% or higher) and with diameters in the range of 60–80 nm
were purchased from SkySpring Nanomaterials, Inc. (Houston, TX, USA).
Polyethylene glycol with a molecular mass of 400 g mol–1 (PEG-400), glutaraldehyde (25% solution in water), mineral oil,
and sorbitan sesquioleate were all provided by Sigma-Aldrich (Burlington,
MA, USA). n-Hexane (minimum purity of 99%) was purchased
from Scharlab SL (Barcelona, Spain). Dulbecco’s modified Eagle’s
Medium (DMEM), penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL)
solution, and UltraGlutamine 1200 mM (U-Gln) were all purchased from
Lonza (Basel, Switzerland). Alpha modification of minimum essential
medium (α modification with sodium bicarbonate, ribonucleosides,
and deoxyribonucleosides without
from Sigma-Aldrich. HyClone characterized fetal bovine serum (FBS)
was purchased from Cytiva (Marlborough, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!